转基因抗虫大豆MON87701品系实时荧光PCR检测方法的建立  

Establishment of Event Specific Real-time PCR Method for Detection of Genetically Modified Soybean MON87701

作　　者：付海滨[1,2] 张敏[3] 吴渺渺 韩宏乾 陈丽静[3] 于洋[2] 

机构地区：[1]沈阳检验检疫科学研究院,辽宁沈阳110016 [2]沈阳出入境检验检疫局综合技术中心,辽宁沈阳110016 [3]沈阳农业大学,辽宁沈阳110866

出　　处：《辽宁农业科学》2016年第4期23-26,共4页Liaoning Agricultural Sciences

基　　金：沈阳市科技计划项目(项目编号:F16-104-4-00)

摘　　要：建立Taq Man实时荧光PCR反应检测转基因抗虫大豆MON87701品系的鉴定方法,为转基因大豆MON87701提供科学的检测依据。根据转基因抗虫大豆MON87701品系特异基因序列设计引物和Taqman探针,并对该方法的特异性、灵敏度和重复性进行检测。结果表明,本研究设计的引物和探针具有很好的品系鉴定特异性,检测灵敏度可达到0.01%(m/m),检测重复性好。因此,建立的转基因大豆MON87701品系特异Taq Man实时荧光PCR检测方法可快速、准确地鉴定转基因抗虫大豆MON87701品系,可在日常检验中推广应用。In order to provide a basis for detection of genetically modi？ ed soybean strains MON87701 ,a n e vent-specific real-tim e PCR method for the detection of genetically modified soybean MON87701 was establishedin this study. Prim ers and TaqM an probe were designed based on the event-specific sequence ofMON87701 soybean. The specificity, sensitivity and repeatability of the developed method were exam ined, respectively.The results indicate this method had good detection specificity and repeatability for geneticallymodified soybean strains,w ith its sensitivity reaching 0 .0 1 % （ m /m ） . These results showed that the strainspecificreal-tim e PCR method was suitable for the identification of genetically modified soybean MON87701 ,itcan be used to testing genetically modified samples.

关 键 词：转基因大豆 MON87701品系 实时荧光PCR 品系鉴定 

分 类 号：Q789[生物学—分子生物学] S565.1[农业科学—作物学]