rFN/CDH纳米仿生骨膜制备及性能表征  

Preparation and characterization of rFN/CDH nano-mimic periosteum

作　　者：杨晓占[1] 李振声[2] 

机构地区：[1]重庆理工大学光电信息学院,重庆400054 [2]第三军医大学生物医学工程学院,重庆400038

出　　处：《功能材料》2016年第8期8120-8123,共4页Journal of Functional Materials

基　　金：重庆市自然科学基金计划资助项目(cstc2012jjA10150);重庆市教委科学技术研究资助项目(KJ130821);国家自然科学基金资助项目(81000670)

摘　　要：骨膜在骨再生和骨修复过程中起到重要的辅助作用,能加速骨缺损愈合。本实验用静电纺丝技术制备双层仿生骨膜,外膜为高分子量壳聚糖(Chitosan);内膜为加入融合蛋白rFN/CDH的低分子量壳聚糖。SEM结果显示双层骨膜都具有类似细胞外基质(ECM)的疏松多孔网状结构。外膜纤维直径为(623±47)nm,内膜纤维直径为(390±53)nm。接触角分析显示内膜材料表面能明显高于外膜;模拟体液(SBF)浸泡表明内膜材料的降解速度快于外膜材料;MSCs细胞培养显示rFN/CDH能明显促进MSCs增殖分化。结果表明该双层仿生骨膜有望在骨膜缺失的骨缺损修复中发挥很好的辅助作用。The periosteum plays an important role in bone regeneration and bone repair. In this study , we createda novel, double-layered,periosteum-mimetic, in vitro. The outer periosteal layer was prepared by usinghigh molecular weight chitosan , while the inner layer w as made from a combination of low molecular weight chitosanand r FN / CD H protein. The SEM photos revealed that both the inner and outer layers have the similarstructure of the ECM which is porous. The SEM also showed that the diameter of the outer and inner layer fiberswere （623 ± 47） nm and （390 ± 53） nm respectively. Analysis of the contact angles revealed that the surfaceenergy of the inner layer w as higher than that of the outer layer. Moreover, in simulated body fluid （SBF ） soakingexperiments the degradation rate of the inner layer was significantly greater than the outer layer. The resultsof cells culture revealed that the r FN / CDH protein can obviously promote proliferation and differentiationof MSCs . Taken together , these results indicate that this novel periosteum-mimetic maypossess some functionsof natural periosteum , such as promoting bone regeneration and creating aspace for bone repair.

关 键 词：壳聚糖 rFN/CDH 静电纺丝 外膜 内膜 

分 类 号：TB34[一般工业技术—材料科学与工程] R318.08[医药卫生—生物医学工程]