差示分光光度法测定漾濞泡核桃花中的总黄酮含量  

The Determination of the Content of Total Flavonoids in Juglans sigillata Dode by Differential Spectrophotometry

作　　者：苏敏 杨盛春 周萍 

机构地区：[1]大理大学药学与化学学院,云南大理671000

出　　处：《大理大学学报》2016年第8期15-19,共5页Journal of Dali University

基　　金：大理大学应用开发研究基金资助项目(KYYY201401)

摘　　要：目的优选漾濞泡核桃花总黄酮的提取工艺,建立漾濞泡核桃花总黄酮含量测定的方法.方法：在单因素考察基础上,用正交试验法对漾濞泡核桃花总黄酮的提取工艺优选,并以芦丁为对照品,采用差示分光光度法测漾濞泡核桃花总黄酮含量.结果：漾濞泡核桃花总黄酮最佳提取工艺为：提取溶剂50%乙醇,料液比1：20,沸水加热回流3次,每次40 min;漾濞泡核桃花总黄酮浓度在11~77 μg/mL范围内与吸光度成良好线性关系（r=0.999 3）,平均加样回收率为99.7%,RSD为1.94%,总黄酮的含量为2.16%~2.82%.结论：此方法简单、准确、稳定、可靠,可作为漾濞泡核桃花总黄酮的含量测定.Objective To optimize extraction technology of total flavonoids from the flower of Juglans sigillata Dode and establish itscontent determination method. Methods： On the basis of single factor test,orthogonal experiment was used to to optimize extractiontechnology of total flavonoids from the flower of Juglans sigillata Dode. With Rutin as the reference substance, differentialspectrophotometry method was adopted to determine the content of total flavonids of the flower of Juglans sigillata Dode. Results： Theoptimum extraction conditions were described as follows： ethanol volume scores 50%, the solid / liquid ratio 1：20, 3 times of heatingreflux,and the time duration for each extraction was 40 min. Total flavonoids of the flower of Juglans sigillata Dode showed good linearrelationship in the range of 11~77 μg/mL（r =0.999 3）,the average recovery was 99.7%,RSD=1.94%. The content of total flavonoidswas 2.24%. Conclusion： The method was simple,accurate,stable and reliable, and could be used to measure the content of totalflavones of the flower of Juglans sigillata Dode.

关 键 词：漾濞泡核桃花 差示分光光度法 总黄酮 含量测定 

分 类 号：R284[医药卫生—中药学]