基底前脑巢蛋白阳性胆碱能神经元抗损伤作用  

Damage Resistance of Basal Forebrain Nestin-positive Cholinergic Neurons

作　　者：王缓缓 朱建华 

机构地区：[1]大理大学基础医学院,云南大理671000

出　　处：《大理大学学报》2016年第8期35-38,共4页Journal of Dali University

基　　金：国家自然科学基金资助项目(31160221)

摘　　要：目的:探索基底前脑巢蛋白阳性和阴性胆碱能神经元对神经毒性损伤的不同反应。方法:30只SD大鼠随机分成5个组,即对照组和秋水仙碱Ⅰ、Ⅱ、Ⅲ、Ⅳ组(10、20、50、100μg)。分别经侧脑室注射10μL生理盐水或不同剂量的秋水仙碱,24 h后4%多聚甲醛灌注取脑,冰冻切片,行胆碱乙酰转移酶和巢蛋白免疫组化。计数和分析基底前脑不同区域巢蛋白阳性和阴性胆碱能神经元的数目。结果:侧脑室秋水仙碱注射后,大鼠基底前脑不同区域巢蛋白阳性和阴性胆碱能神经元数目都下降。注射剂量越大,神经元数目下降越明显。与巢蛋白阳性神经元相比,巢蛋白阴性胆碱能神经元下降出现更早,下降更明显。结论:巢蛋白表达可增强基底前脑胆碱能神经元的抗损伤作用。Objective To explore the different responses of basal forebrain nestin positive and negative cholinergic neurons to theneurotoxicity. Methods： 30 SD rats were randomly divided into 5 groups, which are control group and colchicine Ⅰ,Ⅱ,Ⅲ,Ⅳ groups（10, 20, 50, 100 μg）. 10 microliters of normal saline or different doses of colchicine were injectedintracerebroventricularly. 24 hoursafter injection, 4% paraformaldehyde intracardiac perfusion, brain removal, frozen section, choline acetyltransferase and nestinimmunohistochemistry were carried out. Then, the nestin positive and negative cholinergic neurons of different basal forebrain regionswere counted and analyzed. Results： After intracerebroventricular injection of colchicine, the nestin positive and negative cholinergicneurons in different regions of rats＇ basal forebrain decreased. The greater the colchicine injection dose is, the more obvious thedecrease of the neurons. Compared with nestin positive neurons, the nestin negative cholinergic neurons decreased earlier and moresignificantly. Conclusion： the nestin expression can enhance the damage resistant ability of basal forebrain cholinergic neurons.

关 键 词：基底前脑 巢蛋白 胆碱能神经元 神经可塑性 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]