川丹通脉片中丹参酮ⅡА含量测定研究  

Determination of tanshinone ⅡA in Chuandan Tongmai tablets

作　　者：汪涛 胡卫东 李育芳 巴夏玲 程波[2] 

机构地区：[1]解放军武汉疗养院药械科,湖北武汉430074 [2]华中科技大学同济医学院药学院实验室,湖北武汉430030

出　　处：《中国药物应用与监测》2016年第4期214-216,共3页Chinese Journal of Drug Application and Monitoring

基　　金：2015年军队医疗机构制剂标准提高计划项目(14TG0199)

摘　　要：目的:建立川丹通脉片中有效成分丹参酮ⅡА含量测定HPLC方法。方法:选用Agilent Zorbax Eclipse XDB-C_(18)色谱柱(4.6 mm×250 mm,5μm);以甲醇-水(75∶25)为流动相;检测波长:270 nm;流速:1.0 m L·min^(-1);柱温:35℃。结果:HPLC法测定丹参酮ⅡА不存在干扰,丹参酮ⅡА进样量在1.486~44.570μg·m L^(-1)范围内时,线性关系良好;加样回收率为100.1%,RSD为1.37%。结论:该质量控制方法简便、稳定、可靠,可作为川丹通脉片中丹参酮ⅡА的含量测定方法。Objective： To establish a determination method of active ingredient in Chuandan Tongmai tablets. Methods： The Agilent Zorbax Eclipse XDB-C18 column（4.6 mm × 250 mm, 5 μm） was used, with the mobile phase consisted of methanol-water（75∶25）. The detection wavelength was set at 270 nm, velocity of flow was 1.0 m L·min^-1, the column temperature wa 35 ℃. Results： The tanshinone Ⅱ A was not intervened with other impurities in this HPLC method. The calibration curve was linear when the concentrations of tanshinone Ⅱ A were within the range of 1.486 – 44.570 μg·m L^-1. The average recovery was 100.1%（RSD = 1.37%）. Conclusion： The method is simple, stable, reliable, and can be adopted as the quality control method.

关 键 词：川丹通脉片 丹参酮ⅡA 高效液相色谱法 含量测定 

分 类 号：R286.0[医药卫生—中药学] O657.72[医药卫生—中医学]