UPLC-QTOF-MS/MS对痛安注射液指纹图谱中生物碱类化学成分的研究  

Alkaloids Identification of Tong'an Injection Fingerprint with UPLC-QTOF-MS/MS

作　　者：潘有智[1] 秦建平[1] 王宪平[1] 李家春[1] 黄文哲[1] 王振中[1] 萧伟[1] 

机构地区：[1]江苏康缘药业股份有限公司(中药制药过程新技术国家重点实验室),连云港222001

出　　处：《药学与临床研究》2016年第4期281-283,共3页Pharmaceutical and Clinical Research

基　　金：国家"重大新药创制"--现代中药创新集群与数字制药技术平台(2013ZX09402203)

摘　　要：目的：采用液相与四极杆飞行时间串联质谱（UPLC-QTOF-MS/MS）对指纹图谱中色谱峰进行研究,对痛安注射液中的生物碱类化学成分进行定性鉴别。方法：采用Agilent SBC18RRHD（3 mm×100 mm,1.8μm）色谱柱,以乙腈-0.1%甲酸为流动相进行梯度洗脱,流速0.4 m L·min-1,柱温为25℃,进样量为2μL。检测质谱采用电喷雾离子源（ESI）的飞行时间质谱,正离子模式下采集数据,一级质谱质荷比扫描范围m/z 100~1500,二级质谱质荷比扫描范围m/z30~500。结果：痛安注射液的指纹图谱色谱峰中共鉴别出12个成分。结论：本方法快速、准确地鉴定了痛安注射液的指纹图谱中的主要成分,为痛安注射液质量标准的完善提供依据。Objective： To identify the alkaloids of Tong＇an injection fingerprint using ultra-performanceliquid chromatography with quadrupole- time-of-flight mass spectrometry （UPLC- QTOF-MS）. Methods： TheUPLC was conducted with an Agilent SB- C18 RRHD column （3mmx 100 mm, 1.8 ^ m） and a gradient solventsystem consisting of acetonitrile （A）-0.1% formic acid （adding ammonium acetate 10 mmol per 1000 mL）.The flow rate was 0.4m L-min_1, the column temperature was maintained at 25 ^ , the injection volume was2 ^ L . QTOF-MS and electrospray ion （ESI） source were applied for the qualitative analysis under the positiveion mode, with the MS full mass scan ranged from 100 m/z to 1500 mJz and the MS2 full mass scanranged from 30 m/z to 500 m/z. Results： Twelve major components of fingerprint chromatography were identified.Conclusion： The method established is simple and rapid in components analysis of fingerprint.The study can provide useful information for the perfection of quality standard of Tong＇an Injection.

关 键 词：痛安注射液 UPLC-Q-TOF MS/MS 指纹图谱 化学成分 

分 类 号：R284.1[医药卫生—中药学]