新生牛血中Cu/Zn-SOD的提纯及肝素、壳聚糖的逐层自组装包裹  

Separation and Purification of Cu/Zn-SOD from Newborn Bovine Blood and Prepatation of HEP and CHI Using Layer-by-layer Self-assembly Technique

作　　者：侯静[1] 林春丽 白志军[1] 魏建民[1] 张永明 张峰[1] 

机构地区：[1]内蒙古农业大学生命科学学院,呼和浩特010018 [2]内蒙古维克生生物科技有限公司,呼和浩特010015

出　　处：《中国畜牧兽医》2016年第8期2032-2037,共6页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(21171086;81160213);内蒙古自治区草原英才工程项目(108-108038)

摘　　要：试验旨在通过对超氧化物歧化酶(super oxide dismutase,SOD)改性,提高其稳定性。本试验采用了热变性提取方法从新生牛血中提取Cu/Zn-SOD,采用逐层自组装技术,以肝素与壳聚糖为材料,对从新生牛血中分离提纯的SOD进行物理包裹试验,并对包裹后Cu/Zn-SOD的稳定性进行研究。结果显示,在耐热稳定性上,包裹的SOD相对酶活性比天然SOD提高了3.3倍;在耐酸碱性方面,在pH分别为3.0、11.0的条件下,包裹的SOD相对酶活性较天然SOD提高了2.0倍;在抗胰蛋白酶水解的能力上,包裹的SOD相对酶活性较天然SOD提高了2.7倍。采用肝素、壳聚糖制成的外壳对SOD进行物理修饰可得到较好的效果,且其具有较强的机械强度、生物相容性较好,外表面的壳聚糖有助于帮助跨细胞膜转运,可应用于口服剂型SOD的开发。In order to improve the stability of super oxide dismutase（SOD）with some modification,we used thermal denaturation extraction method to extract Cu/Zn-SOD from newborn bovine blood,and used layer-by-layer self-assembly technique,heparin and chitosan as material to modificate the SOD which separated and purified from newborn bovine blood.The results showed that compared with the natural SOD,the modified SOD increased 3.3times in the thermal stability;In acid and alkaline,under the condition of pH 3.0and pH 11.0,the acid-base resistance property of the modified SOD raised 2.0times;On the antitrypsin hydrolysis ability,the relative enzyme activity of modified SOD had increased 2.7times than natural SOD.The modified SOD with shell outside made of heparin and chitosan by physical modification could get better effect,and had the strong mechanical strength,better biocompatible,outer surface of chitosan was helpful to cross the cell membrane transport,and it could be applied to the development of oral dosage forms of SOD.

关 键 词：SOD 肝素 壳聚糖 逐层自组装技术 相对酶活性 

分 类 号：S852.5[农业科学—基础兽医学]