中药野菊花提取物与抗菌药联合对产ESBLs大肠杆菌抑制效果研究  

Antibacterial Effect of Aqueous Extract of Chrysanthemum indicum L.Combined with Antibiotics on Extended Spectrumβ-lactamases-producing Escherichia coli

作　　者：孙燕杰[1] 吴永继 刘增援 胡梅[1] 赵雨川 黄凯[1] 司红彬[1] 

机构地区：[1]广西大学动物科学技术学院,南宁530005

出　　处：《中国畜牧兽医》2016年第8期2170-2175,共6页China Animal Husbandry & Veterinary Medicine

基　　金：国家自然科学基金(31460675);广西自然基金面上项目(2013GXNSFAA019070);南宁市科技攻关项目(20141295);广西大学大学生创新训练项目(140)

摘　　要：试验旨在探讨野菊花提取物与抗菌药联合作用对产超广谱β-内酰胺酶(extended spectrumβ-lactamases,ESBLs)大肠杆菌的体外抑制效果,为野菊花的进一步开发利用提供理论依据。采用2倍微量稀释法和中西药联合诱导传代法测定野菊花提取物、抗菌药及联合作用后的最小抑菌浓度(minimal inhibitory concentration,MIC)。结果显示,野菊花水提物对该耐药大肠杆菌的MIC值为250mg/mL,且在野菊花水提物亚抑菌浓度(1/2 MIC)作用下进行中西药联合传代,其与头孢曲松钠、头孢噻呋钠、头孢噻肟钠、氟苯尼考、痢菌净、阿米卡星、林可霉素7种抗菌药联合作用后,抗菌药的MIC值明显降低。表明野菊花水提物能够显著降低产ESBLs大肠杆菌对部分抗菌药的敏感性。The experiment was done to investigate the antibacterial effect of aqueous extract from Chrysanthemum indicum L.combined with antibiotics on extended spectrum β-lactamases（ESBLs）-producing E.coli,and aimed to offer theoretical basis for its further development.The ESBLs-producing E.coli was induced to passage by combining Chinese herbal medicine with antibacterial drugs.Then the minimal inhibitory concentration（MIC）of medicines were determined with double microdilution method.The results showed that the MIC of Chrysanthemum indicum L.aqueous extract was 250mg/mL.And the MIC of ceftriaxone,ceftiofur sodium,cefotaxime,florfenicol,mequindox,amikacin and lincomycin decreased significantly after combining with 1/2MIC of Chrysanthemum indicum L.aqueous extract.This research showed that aqueous extract Chrysanthemum indicum L.could increase the antibiotic sensitivity of the ESBLs-producing E.coli.

关 键 词：野菊花 超广谱Β-内酰胺酶 大肠杆菌 联合传代 抑菌效果 

分 类 号：S853.7[农业科学—临床兽医学]