盾壳霉抗逆性菌株的筛选  

Breeding of stress resistance mutants about bio-control fungi Coniothyrium minitans

作　　者：金强[1] 段作营[2] 夏森玉 沈志松 李华钟[2] 金坚[1] 

机构地区：[1]江南大学药学院,江苏无锡214122 [2]江南大学工业微生物技术教育部重点实验室,江苏无锡214122 [3]无锡楗农生物科技有限公司,江苏无锡214122

出　　处：《工业微生物》2016年第4期8-13,共6页Industrial Microbiology

基　　金：2015江苏省重点研发计划(现代农业)重点项目(BE2015309)

摘　　要：通过测定野生型盾壳霉JN-CM菌株对所选的各种农药和化肥的耐受性,确定草甘膦、吡虫啉和磷肥对盾壳霉具有较大的抑制作用。采用紫外诱变方式分别筛选了对草甘膦、吡虫啉和磷肥具有一定耐受性的突变菌株,分别命名为UV-G(草甘膦抗性)、UV-I(吡虫啉抗性)和UV-P(磷肥抗性)。此3种抗性突变株具有良好的遗传稳定性,同时在生长繁殖能力及侵染相关酶活性方面与野生菌株无明显差异,具有良好的应用潜力。Tolerance of wild-type Coniothyrium minitans strain JN-CM were determined to selected pesticides and fertilizer. The results indicated that glyphosate, imidacloprid and phosphate fertilizer had significant inhibitory effects on the strain. Then the mutant stain with a certain degree of tolerance of glyphosate, imidacloprid and phosphate fertilizer were successfully screened through ultraviolet mutagenesis and named as glyphosate （ UV-G） , imidacloprid （ UV-I） and phosphate fertilizer （ UV-P ） , respectively. Three mutant strains had good genetic stability. Mutants had no significant differences between ability of growth and infection-associated enzyme activity if compared with those of wild-type strain, which showed their good application prospects.

关 键 词：盾壳霉 突变菌株 紫外诱变 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]