动脉粥样硬化大鼠心肌梗死时钙敏感受体表达的变化及其作用机制  

The Change and Mechanism of Calcium Sensing Receptor Expression in Atherosclerosis Rat combined with Myocardial Infarction

作　　者：郭津[1] 顾镜月[2] 王鲁川[2] 吕洋[1] 许洪伟[1] 孙颖[1] 徐长庆[3] 李鸿珠[3] 张士岭[1] 马冬梅[1] 

机构地区：[1]佳木斯大学附属第三医院小儿神经科,黑龙江佳木斯154002 [2]佳木斯大学附属第一医院儿一科,黑龙江佳木斯154003 [3]哈尔滨医科大学基础医学院病理生理教研室,黑龙江哈尔滨150086

出　　处：《现代生物医学进展》2016年第24期4631-4635,4679,共6页Progress in Modern Biomedicine

基　　金：黑龙江省教育厅科学技术研究项目(12521522)

摘　　要：目的:探讨动脉粥样硬化大鼠心肌梗死时钙敏感受体表达的变化及其作用机制。方法:Wistar大鼠随机分为4组:正常对照组(Control);异丙肾上腺组(ISO);动脉硬化组(AS);动脉硬化+异丙肾上腺素组(AS/ISO)。采用腹腔注射VD3和高脂饮食及大剂量异丙肾上腺素(ISO 200 mg/kg)皮下注射复制大鼠在体动脉粥样硬化心肌梗死模型,应用RT-PCR测定心肌组织中Ca SR、Bax、Bcl-2和caspase-3 m RNA的表达变化;TUNEL染色观察心肌细胞凋亡情况;光镜观察心肌形态学变化;紫外分光法检测血清肌酸激酶(CK)、电化学免疫发光法检测肌钙蛋白T(c Tn T)水平。结果:与正常组比较,ISO组CK活性、c Tn T水平以及Ca SR、Bax和caspase-3的表达均增加,同时Bcl-2表达减少,心肌细胞损伤严重,AS/ISO的心肌损伤进一步加重。结论:Ca SR的表达增多参与了动脉硬化大鼠心肌梗死的发生,其机制可能与促进心肌细胞凋亡有关。Objective： To investigate the change and mechanism of calcium sensing receptor expression in atherosclerosis rat combined with myocardial infarction. Methods： A rat atherosclerosis model combined with myocardial infarction was established, in which rats received a single dose of vitamin D3 and were fed a high-fat and received isoproterenol （ISO） treatment, ISO was used to induce myocardial infarction. The rat myocardial infarction models were prepared using subcutaneous injection ofisoprel （200 mg·kg^-1·d^-1 ）.Wistar rats were divided into four groups randomly： ①Control group; ②ISO group; ③AS group;④AS/ISO group. The expressions of CaSR, Bcl-2, Bax and caspase-3 mRNA were analyzed using RT-PCR respectively. Apoptotic cells were measured by TUNEL staining assay. The morphological changes of cardiac tissues were observed under optical microscope. The activity of creatine kinase （CK）, were assayed with ultraviolet spectrophotometer. The level of cTnT was detected by electrochemical immunofluorescence. Results： Compared with the control group, the activity of CK and cTnT, apoptosis index, expression of CaSR, Bax and caspase-3 were increased, but the Bcl-2 expression was decreased, the myocardial ultrastructure injury was aggravated in the ISO group. The further increased of above parameters in AS/ISO group. Conclusions： The increased expression of CaSR was involved in the pathogeneisi of atherosclerosis combined with myocardial infarction, which might be related with and the increase of apoptosis.

关 键 词：钙敏感受体 心肌梗死 细胞凋亡 动脉硬化 

分 类 号：R33[医药卫生—人体生理学] R542.22[医药卫生—基础医学]