草地早熟禾叶绿素酶1基因PpCHL1的克隆和表达分析  

Cloning and Expression Analysis of Chlorophyllase 1 Gene PpCHL1 from Poa pratensis L.

作　　者：张兰[1] 滕珂[1] 尹淑霞[1] 

机构地区：[1]北京林业大学草坪研究所,北京100083

出　　处：《中国草地学报》2016年第4期1-7,共7页Chinese Journal of Grassland

基　　金：国家自然科学基金项目"空间诱变草地早熟禾矮化突变体矮化机理研究"(31302016)

摘　　要：利用RACE技术首次从草地早熟禾中克隆出叶绿素酶1基因(PpCHL1)的全长cDNA序列,提交到GenBank,登录号为KU145126;其开放阅读框为951bp,编码361个氨基酸,等电点6.11,平均亲水指数0.084,属于疏水性酸性蛋白。高级结构分析表明,其具有脂肪酶家族的保守域及水解酶特异的功能活性位点。利用实时荧光定量PCR分析其在草地早熟禾根、茎和叶及三种植物生长调节剂脱落酸(ABA)、水杨酸(SA)和茉莉酸甲酯(MeJA)处理后的表达情况,结果显示PpCHL1基因在草地早熟禾不同组织的表达情况存在明显的组织差异性,叶中的表达量最高且受ABA、SA和MeJA诱导。The full length cDNA encoding chlorophyllase1（PpCHL1）was cloned from Poa pratensis through the methods of RACE technologies.The PpCHL1 gene was submitted to GenBank and obtained the accession number KU145126.For PpCHL1,the open reading frame comprised 951 bp,encoding 361 amino acid,isoelectric point was 6.11 and grand average of hydropathicity was 0.084,which means the PpCHL1 was Hydrophobic acid protein.Advanced structure analysis showed that PpCHL1 gene included family Esterase-lipase abhydrolase-5conserved domains and hydrolase specific functional active sites.The expression level of PpCHL1 gene was analyzed in different tissues of root,stem and leaf,as well as under the treatment of three plant hormones ABA,SA and MeJA applications by real-time-fluorescence quantitative PCR.The results showed that expression level of PpCHL1 was different among root,stem and leaf,which the expression level in leaf was the highest.Moreover,the expression of PpCHL1 was induced by ABA,SA and MeJA.

关 键 词：草地早熟禾 叶绿素酶1 克隆 序列分析 表达分析 

分 类 号：S688.4[农业科学—观赏园艺]