锰胁迫对紫花苜蓿幼苗碳、氮代谢的影响  

Effect of Manganese on the Carbon-nitrogen Metabolism of Medicago sativa Seedlings

作　　者：吴旭红[1] 张超[1] 马云祥[1] 何旻琦 

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006

出　　处：《中国草地学报》2016年第4期49-54,共6页Chinese Journal of Grassland

摘　　要：采用沙培法,以常规锰浓度为对照,研究不同锰营养状况对苜蓿早期光合碳同化与氮代谢的影响,结果表明:锰素缺乏(T1处理:5%CK;T2处理:20%CK),苜蓿叶片1,5-二磷酸核酮糖羧化酶积累量减少,光合色素含量下降,硝酸还原酶和谷氨酰胺合成酶活性降低。锰重度过量(T4处理:20倍CK),苜蓿幼苗碳氮代谢运转受阻,可溶性蛋白、氨基酸总量和羧肽酶活性大幅下降。5倍常规浓度的中度锰过量处理,则促进了碳素同化、氨基酸的合成和可溶性蛋白的积累。综合分析表明,中度锰过量有助于苜蓿苗期的光合碳同化和蛋白质代谢。Alfalfa was cultivated with sand culture method,and general Mn concentration was used as control,a series of experiments were designed to explore the function and effect of Manganese on the carbon-nitrogen metabolism of Medicago sativa seedlings.The results showed that when the manganese was deficient（T1：5%CK;T2：20%CK）,the accumulation amount of Rubisco reduced,the content of photosynthetic pigment decreased,and the activities of NR and GS declined.On the other hand,when the Manganese was overfeeding seriously（T4：20times of control）,the carbon-nitrogen metabolism would be inhibited,the soluble protein,total amino acids and the activity of Rubisco decreased greatly.However,under light stress which were 5times of general Mn concentration,it would be benefit to the Medicago sativa seedlings on the protein metabolism and photosynthesis.In conclusion,aproper overfeeding of Manganese would promote the carbon-nitrogen metabolism and protein metabolism in Medicago sativaseedlings.

关 键 词：锰胁迫 苜蓿幼苗 碳氮代谢 1 5-二磷酸核酮糖羧化酶 谷氨酰胺合成酶 

分 类 号：Q945.78[生物学—植物学]