紫草素对鼠类繁殖的影响  

Effect of Shikonin on Mice Reproduction

作　　者：包达尔罕 满都呼[1,2] 袁帅[1,2] 武晓东[1,2] 付和平[1,3,2] 

机构地区：[1]内蒙古农业大学生态环境学院,内蒙古呼和浩特010019 [2]草业与草地资源教育部重点实验室,内蒙古呼和浩特010019 [3]内蒙古农业大学职业技术学院,内蒙古包头014109

出　　处：《中国草地学报》2016年第4期99-104,共6页Chinese Journal of Grassland

基　　金：国家自然科学基金项目(31260580;31560669);内蒙古自然科学基金项目(2014MS0325);农业部公益行业项目(201203041)

摘　　要：为明确植物提取物紫草素的抗生育作用,以昆明小白鼠为实验对象,进行繁殖器官检测和雌、雄处理实验。结果表明:50mg/kg浓度紫草素实验组的雌性子宫脏器系数极显著低于对照组和其他两个浓度实验组(F=6.29,P<0.01),子宫出现明显萎缩,雄性精囊腺脏器系数、精子密度均显著低于对照组(F=6.49,P<0.01;F=4.60,P<0.05),50mg/kg浓度紫草素具有抗生育的预期效果;50mg/kg浓度紫草素显著降低了雌性处理组和雌、雄处理组的出生率,而对雄性处理组的作用不显著,但雌性和雄性的繁殖启动期均显著延迟,有效降低了小鼠整个繁殖期的繁殖率。因此,紫草素可作为一种潜在的鼠类不育控制剂应用于小型鼠类不育控制。The study was to observe the anti-fertility effects of plant extract shikonin.Kunming mice were used as the test subjects in the experiment.The reproductive organs of the mice treated with 5mg/kg,20mg/kg,and 50mg/kg of shikonin were detected.The female uterus,male seminal vesicle,testicle and sperm density were anatomized and measured in viscera coefficients.And an ideal shikonin concentration of 50mg/kg was selected for breeding experiments.The results showed that the female uterus viscera coefficient of the mice from the group treated with a shikonin concentration of 50 mg/kg was significantly lower than that of the control group and the other two concentration groups（F=6.29,P 〈 0.01）,and the female uterus showed obvious atrophy.The male seminal vesicle viscera coefficients and sperm density of the mice from the group treated with a shikonin of 50 mg/kg was significantly lower than the control group（F=6.49,P 〈 0.01;F=4.60,P 〈0.05）.A shikonin concentration of 50mg/kg had the expected anti-fertility effects.Results of mice reproduction experiments showed that a shikonin concentration of 50 mg/kg significantly reduced the birth rate of the female treatment and the male-female treatment groups,however,the effects on the male treatment group was not significant.Nevertheless,the reproductive start-up period of female and male mice was significantly delayed,which would effectively reduce the reproduction rate during mice breeding periods.Therefore,shikonin can be used as a potential fertility control agent for small rodents.

关 键 词：植物提取物 紫草素 抗生育 

分 类 号：S812.6[农业科学—草业科学]