小檗碱对颅脑创伤模型小鼠对侧顶叶皮层继发性损伤的影响  

Effects of berberine chloride on secondary brain injury in contralateral parietal lobe cortex of TBI model mice

作　　者：黄树宣 朱飞奇 裴中[3] 朱瑾华[1] 杨志[1] 邓旭辉[1] 刘远[1] 

机构地区：[1]汕头大学医学院附属粤北人民医院神经内科,韶关512026 [2]深圳罗湖区人民医院神经内科 [3]中山大学附属第一医院神经科

出　　处：《中国神经精神疾病杂志》2016年第6期338-341,共4页Chinese Journal of Nervous and Mental Diseases

基　　金：广东省自然科学基金面上项目(编号:S2013010015786);韶关市科技计划项目[编号:韶科(卫)2010-01]

摘　　要：目的观察小鼠颅脑创伤(traumatic brain injury,TBI)后对侧皮层的炎症反应、氧化损伤及神经元丢失的情况,并探讨盐酸小檗碱对这种继发性损害的影响。方法采用自由落体撞击法建立TBI小鼠模型,通过灌胃给予盐酸小檗碱[50mg/(kg·d)],共21 d。采用免疫荧光染色观察对侧顶叶皮层小胶质细胞、星形胶质细胞的增殖活化情况,免疫组化染色观察对侧皮层COX-2(cyclooxygenase-2)、i NOS(inducible nitric oxide synthase)的表达情况以及DNA氧化损伤和神经元丢失的情况。结果 TBI导致对侧顶叶皮层小胶质细胞和星形胶质细胞的增殖活化、COX-2和i NOS的表达以及DNA氧化损伤较对照组明显增加,分别为(19.82±1.88)和(16.96±1.69)、(13.79±4.32)和(8.67±0.96)、(27.86±5.38)和(16.00±7.59)、(31.92±6.57)和(24.79±2.78)(P<0.01或P<0.05),但神经元数量无显著丢失,分别为(49.05±4.38)和(48.56±3.56)(P>0.05);小檗碱显著抑制对侧皮层小胶质细胞、COX-2和i NOS的表达,分别为(15.49±1.88)和(19.82±1.88)、(16.83±7.89)和(27.86±5.38)、(26.25±2.41)和(31.92±6.57)(P<0.01或P<0.05),而星形胶质细胞和DNA氧化损伤的比较差异无统计学意义,分别为(11.91±1.42)和(13.79±4.32)、(32.82±2.78)和(34.40±2.41)(P>0.05)。结论 TBI导致远隔对侧顶叶皮层继发性损害较轻,仅导致炎症反应、氧化损伤增加,但并不会导致远隔对侧皮层神经元丢失;盐酸小檗碱能够显著抑制TBI后对侧皮层的炎症反应。Objective To examine neuroinflammation,oxidative damage and neuron loss in the contralateral parie-tal lobecortex of TBI model mice, and to investigate effects of berberine chloride on such secondary damage.Methods TBI model was established by a weight-drop hitting device and mice in berberine group were administered intragastrically with berberine chloride （50mg/kg.day） for 21 days.Immunofluorescence staining was used to assess activity of microglia and astrocyte.Immunohistochemistry was used to assess DNA oxidative damage, neuron loss and expression of COX-2 and iN-OS.Results Activation of microglia and astrocyte, expressions of COX-2 and iNOS and DNA oxidative damage were ob-viously increased by TBI,（19.82 ±1.88）and（16.96 ±1.69）、（13.79 ±4.32）and（8.67 ±0.96）、（27.86 ±5.38） and （16.00 ±7.59）、（31.92 ±6.57）and（24.79 ±2.78）respectively （P〈 0.01 or P〈 0.05）.Activation of microglia and ex-pressions of COX-2 and iNOS were significantly suppressed by berberine ,（15.49 ±1.88）and（19.82 ±1.88）、（16.83 ± 7.89）and（27.86 ±5.38）、（26.25 ±2.41）and（31.92 ±6.57） respectively（P〈 0.01 or P〈 0.05）.There was no differ-ence in neuron loss among three groups, （49.05 ±4.38）,（48.56 ±3.56）and （47.75 ±4.14） respectively （P〉0.05）.Conclusions TBI can cause neuroinflammation and oxidative damage but not neuron loss in the contralateral parietal lobe cortex.Berberine chloride can significantly suppress neuroinflammtion in the contralateral parietal lobe cortex after TBI.

关 键 词：颅脑创伤 盐酸小檗碱 对侧皮层 炎症反应 氧化损伤 神经元丢失 

分 类 号：R651.15[医药卫生—外科学] R-332[医药卫生—临床医学]