高分子生物复合膜作为微粒皮移植覆盖物的实验研究  

An experimental study of bio-polymer composite film as a micro-skin autograft covering

作　　者：解英[1] 冷崇燕[2] 汪虹[1] 曹文德[1] 李武全[1] 彭颖[1] 赵阳[1] 

机构地区：[1]昆明医科大学第二附属医院,650000 [2]昆明理工大学材料科学与工程学院,650000

出　　处：《实用医学杂志》2016年第16期2617-2621,共5页The Journal of Practical Medicine

基　　金：云南省科技计划项目(编号:2014NS065)

摘　　要：目的：探讨高分子生物复合膜作为微粒皮移植覆盖物对创面愈合的促进作用。方法：30只实验家兔背部两侧制作全层皮肤缺损模型,将自体微粒皮移植于创面后,采用自体对照,随机分为实验侧和对照侧,实验侧予壳聚糖/葡甘聚糖复合膜覆盖、对照侧予脱细胞猪皮覆盖,术后1、2、3、4、5周创面取材,观察创面愈合情况、测定创面愈合率、HE染色、免疫组化检测PCNA及CD31。结果：（1）术后2~4周实验侧创面愈合率显著高于对照侧（P〈0.01）;（2）术后实验侧炎性细胞浸润量少于对照侧;（3）术后1~2周实验侧PCNA表达高于对照侧（P〈0.01）,术后3~4周实验侧PCNA表达低于对照侧（P〈0.01）;（4）术后1~5周实验侧CD31表达均高于对照侧（P〈0.01）。结论 ：壳聚糖/葡甘聚糖复合膜作为微粒皮移植覆盖物可促进创面愈合。Objective To explore the promoting effects of bio-polymer composite film as a micro-skin autograft covering on wound healing. Methods The full thickness skin defect models were made on both sides of 30 experimental rabbits. Then, the rabbits were randomly divided into experimental group and control group. In the former group, the side was covered with chitosan / glucomannan composite membrane and in the latter, the side covered with acelluar porcine skin after micro-skin autograft. We obtained wound tissues at week 1, 2, 3, 4 and 5 after operation. The conditions of wound healing were observed, the rate of wound healing was calculated, HE staining was made, and PCNA and CD31 were detected by immunohistochemistry. Results（1） During 2 ~ 4 weeks after operation, the rate of wound healing in the experimental group was significantly higher than that of the control wound（P 0.01）.（2） The amount of neutrophil in experimental group was less than that of the control after operation.（3） During 1 ~ 2 weeks after operation, the expression of PCNA in the experimental group was higher than that of the control group（P 0.01）, but lower than the control wounds during 1 ~ 2 weeks after operation（P 0.01）.（4） During 1 ~ 5 weeks after operation, the expression of CD31 in the experimental group was higher than that of the control group（P 0.01）. Conclusion Chitosan / glucan-mannan composite membrane as a micro-skin autograft covering may promote wound healing.

关 键 词：微粒皮移植 高分子生物复合膜 脱细胞猪皮 

分 类 号：R644[医药卫生—外科学]