孕妇外周血中有核红细胞数与妊娠期高血压疾病的关系  

To Predict Hypertention by Pregnancy in Counting Nuclear Red Blood Cells in Peripheral Blood

作　　者：刘丽平[1] 龚瑞龙 周洁琼[1] 戴翔[1] 宋晓婕[1] 

机构地区：[1]湖北省武汉市妇女儿童医疗保健中心妇产科,430016

出　　处：《实用医学杂志》2016年第16期2649-2651,共3页The Journal of Practical Medicine

基　　金：武汉市卫计委2014年立项课题

摘　　要：目的：通过比较正常孕妇和妊娠期高血压疾病孕妇外周血中胎儿有核红细胞数量,了解孕妇外周血中胎儿有核红细胞数量变化与妊娠期高血压疾病之间的关系。方法：收集69例孕12~18周孕妇外周血,采用密度梯度离心法分离外周单个核细胞后使用PE-GPA/FITC-CD71单克隆抗体标记胎儿有核红细胞后进行流式细胞计数,分析其与妊娠期高血压疾病之间的关联性。结果：妊娠期高血压疾病患者外周血中胎儿有核红细胞较正常妊娠者增多,对照组和病例组中GPA＋/CD71＋百分比分别为（0.89±0.81）%和（3.57±3.48）%,其差异具有统计学意义（P〈0.05）。结论 ：妊娠期高血压疾病患者外周血中胎儿有核红细胞增多,孕妇外周血中胎儿有核红细胞增加提示有患妊娠期高血压疾病的可能,应积极采取干预措施。Objective To predict the Hypertention by pregnancy in counting nuclear red blood cells peripheral blood. Methods Blood samples were obtained from 69 pregnant women with the gestational age from 12 to18 weeks. Peripheral blood mononuclear cells were isolated by density gradient centrifugation and then analyzed the cells marked with PE-GPA / FITC-CD71 monoclonal antibodies on the flow cytometer. Correlations between them were analyzed after tracking of hypertention by pregnancy. Results Percentage of GPA＋ / CD71＋ cells in healthy pregnant women was 0.89 ± 0.81%, while that in pregnant women with hypertention was 3.57 ± 3.48%. There was a significant difference between the two groups（P 0.05）, and the percentage of GPA＋ / CD71＋ cells in the group of hypertention by pregnancy was much higher than that in the healthy group. Conclusions The increased nuclear red blood cells in maternal peripheral blood related to hypertention by pregnancy. The counting of nuclear red blood cells in maternal peripheral blood have potential value to predict hypertention by pregnancy.

关 键 词：胎儿有核红细胞 流式细胞术 妊娠期高血压疾病 

分 类 号：R714.246[医药卫生—妇产科学]