血管紧张素原基因多态性与低氧习服效果的关联研究  

Studies on the association of angiotensinogen polymorphisms and hypoxia acclimatization

作　　者：潘秀清[1] 胡扬[2] 徐飞[3] 

机构地区：[1]西安体育学院健康科学系,陕西西安710068 [2]北京体育大学研究中心,北京100084 [3]浙江工业大学体育科学研究所,杭州310023

出　　处：《中国应用生理学杂志》2016年第4期314-318,共5页Chinese Journal of Applied Physiology

基　　金：北京市教委重点实验室开放性课题

摘　　要：目的:研究血管紧张素原基因(AGT)G-217A和T174M两个位点的多态性与急性高原反应(AMS)的发生及其低氧习服效果的关系。方法:阶段1:61名北方汉族大学生,在低氧室急性低氧暴露6 h(模拟海拔4 800 m),入室后先安静休息30 min,再仰卧蹬车20 min,蹬车负荷定量为60 r/min、80 W,用路易斯湖评分系统(LLS)评价AMS,并记录运动过程中HR、动态血压、SpO_2等生理指标的值;阶段2:进行3周模拟低氧训练,氧含量分别相当于海拔2 500 m、3 500 m、4 800 m,同时以中等强度负荷量运动,2 h/d、4 d/周。3周后,再以阶段1的试验条件测试相应指标;采用PCR-RFLP法检测受试者AGT基因G-217A和T174M位点的基因型和等位基因频率。结果:第1次低氧暴露,在AGT基因的G-217A位点上,GG与GA+AA基因型受试者的各项生理指标无显著性差异;第2次低氧暴露,GG基因型受试者的SpO_2明显低于GA+AA基因型(P<0.05);T174M位点的不同基因型和等位基因携带者在2次暴露中其AMS发生率、VE、SpO_2、HR和血压等生理指标均无显著性差异。结论:G-217A位点可能是低氧习服的遗传学标记;T174M位点的多态性与AMS的发生及低氧习服未见明显关联。Objective： To research the effects of gene polymorphisms of AGT at G-217A and T174M loci on inc, idence and the hypoxia acclimation to acute mountain sickness （AMS）. Methods： Step 1 ： 61 Han students origin low landers were exposed in acute hypoxia at 4 800 m altitude-equivalent for 6 hours, and took the supine bicycle exercise for 20 min at quantitative load of 60 r/min, 80 W after entering the cabin for 30 min, then the AMS were evaluated by the Lake Louise acute mountain sickness scoring system （MS）. The physical index of hem＇t rate （HR）, ambulatory blood pressure and oxygen saturation （SpO2） were measured during exercise. Step 2： The physical index was measured again in acute hypoxic exposure after 3 weeks increasing hypoxia exercise （At an increasing altitude of 2 500 m, 3 500 m, 4 800 m, time of 2 hours/day, 4 days/week, and in moderate intensity of exercise）. PCR-RFLP was used to detemfine the genotypes and alleles frequencies of AGT at G-217A and T174M loci. Results： In step 1 the hypoxic exposure, for the G-217A locus in AGT, there was no significant difference between GG and GA ＋ AA gene tester. While in step 2 the hypoxic exposure, the SpO2 of GG gene was lower than GA ＋ AA gene obviously （ P 〈 0.05）. No significant differences of AMS incidence, VE, SpO2, HR and blood pressure were detected in different genotypes and alleles at T174M locusin both hypoxic exposures. Conclusion： The G-217A may be the genetics sign of hypoxia acclimation. There is no obvious rel- evancy between the TI74M polymorphism and the occurrence of AMS/hypoxia acclimation.

关 键 词：急性高原反应 基因多态性 低氧习服 AGT 

分 类 号：G804.7[文化科学—运动人体科学]