四妙勇安汤中甘草苷、金丝桃苷和木犀草苷提取率的拆方分析  

Decomposition analysis on extraction rate changes of liquiritin,hyperoside and luteoloside in Simiao Yong'an Decoction

作　　者：胡少伟[1] 姜艳艳[1] 高尧春 王翀[1] 陈路晓 刘斌[1] 

机构地区：[1]北京中医药大学中药学院,北京100102

出　　处：《北京中医药大学学报》2016年第7期590-594,共5页Journal of Beijing University of Traditional Chinese Medicine

基　　金：北京市建委共建科研基地建设项目

摘　　要：目的研究四妙勇安汤的配伍组合对甘草苷、金丝桃苷和木犀草苷提取率的影响。方法将四妙勇安汤拆方,采用HPLC法,SunfireTMC18色谱柱(4.6 mm×150 mm,5μm),流动相为乙腈-0.1%磷酸等梯度洗脱,流速1.0 m L/min,检测波长276 nm(甘草苷)、350 nm(金丝桃苷、木犀草苷),柱温35℃,分析各组合样品中甘草苷、金丝桃苷和木犀草苷含量并计算提取率。结果与单味药相比,配伍药味越多,甘草苷、金丝桃苷和木犀草苷的提取率越高,全方合煎后,3种成分提取率均达到最高。结论配伍可以明显提高四妙勇安汤中甘草苷、金丝桃苷和木犀草苷的提取率,从化学成分提取率角度验证了复方配伍的科学性和合理性,阐明了中药复方配伍的科学内涵。Objective To investigate the influence of different decomposed combinations of Simiao Yong＇an Decoction（ four-ingredient heahh-restoring decoction） on the extraction rates of liquiritin, hy- peroside and luteoloside. Methods The extraction rates of liquiritin, hyperoside and luteoloside were determined with HPLC using a SunfireTM Cls analytical column （4.6ram x 150ram, 5 p,m） in decomposed formula of Simiao Yong＇ an Decoction. The mobile phase was composed of aqueous phosphoric acid （0.1% ） and acetonitrile using anisocratic elution. The column temperature was 35℃. The flow rate was 1.0 mL/min while the detection wavelength was set at 276 nm （liquiritin）and 350 nm （hyperoside and luteoloside）. The contents and the extraction rates of liquiritin, hyperoside and luteoloside from the origi- nal and dismantled formula were detected and analyzed. Results Compared with the single herb, the more ingredients it contained, the higher extraction rates of liquiritin, hyperoside and luteoloside. When the whole ingredients were stewed together, the extraction rates of these 3 components reached the peak. Conclusion The different decomposed SimiaoYong＇an Decoction can significantly improve the extrac- tion rates of liquiritin, hyperoside and luteoloside, which indicated the validity and rationality of com- pound compatibility from the extraction rates of chemical compositions.

关 键 词：四妙勇安汤 拆方 甘草苷 金丝桃苷 木犀草苷 提取率 

分 类 号：R284.1[医药卫生—中药学]