母乳性黄疸患儿肠道菌群宏基因组学研究  

Metagenome of gut microbiota of patients with breast milk jaundice

作　　者：周建利[1] 周少明[1] 王明帮 程勇卫[1] 代东伶[1] 蔡华波[1] 朱忠生[1] 邹瑜[1] 丁娟[1] 

机构地区：[1]汕头大学医学院附属深圳市儿童医院消化内科,广东深圳518000 [2]深圳市众循精准医学研究院,广东深圳518000

出　　处：《中国微生态学杂志》2016年第8期893-898,共6页Chinese Journal of Microecology

基　　金：深圳市科技创新委员会课题(JCYJ20140416141331514)

摘　　要：目的探讨母乳性黄疸(BMJ)患儿的肠道菌群情况。方法采用鸟枪法宏基因组测序方法,对16例BMJ患儿及16例正常婴儿的肠道菌群进行研究。结果 BMJ组与对照组中样品菌种丰度及菌群种类总的水平上差异无统计学意义(P>0.05);在部分菌种上有差异,双歧杆菌、脆弱拟杆菌及多形拟杆菌在BMJ组中较对照组少(P<0.05);在菌群基因的功能通路上差异也有统计学意义(P≤0.05),尤其是在糖合成和代谢基因功能通路中,BMJ组比对照组表达较少些,差异有统计学意义(P<0.05)。结论 BMJ患儿肠道菌群的种类、丰度与正常同龄婴儿总体上差异不大,在菌种上有部分差异,双歧杆菌、脆弱拟杆菌及多形拟杆菌在BMJ患儿肠道中含量较少,可补充双歧杆菌、拟杆菌制剂使BMJ患儿的肠道菌群平衡。同时,在基因功能通路表达上,BMJ患儿与正常同龄婴儿有显著差别,特别是糖合成和代谢通路,可深入研究。Objective To investigate the gut microbiota of infant patients with breast milk jaundice（BMJ）.Methods Shotgun metagenome sequencing was performed on the gut microbiota of 16 patients with BMJ and 16 normal infants.Results Generally the species and abundance of the gut microbiota in the BMJ group were not statistically different from those of controls（P0.05）.There were differences in some species,for example,the amounts of Bifidobacteria,Bacteroides fragilis and Bacteroides thetaiotaomicronin BMJ group were less than those in the control group（P0.05）.The differences in the functional pathways of the genes of microbiota were also statistically significant（P≤0.05）.Especially in the pathways of glycan biosynthesis and metabolism,BMJ group expressed less genes than controls（P0.05）.Conclusion Generally,no significant differences were seen in the species and abundance of gut microbiota between the BMJ group and control group.The amounts of Bifidobacteria,Bacteroides fragilis and Bacteroides thetaiotaomicronin BMJ group were less.Moreover,in functional genetic pathways,the differences between BMJ group and control group were statistically significant,especially in the pathways of glycan biosynthesis and metabolism.

关 键 词：母乳性黄疸 肠道菌群 宏基因组学 鸟枪法测序 

分 类 号：R725.7[医药卫生—儿科] R378[医药卫生—临床医学]