橄榄苦苷与羟基酪醇的大鼠在体胃肠吸收比较  

Comparison of Gastrointestinal Absorption of Oleuropein and Hydroxytyrosol in Rats

作　　者：赵鑫[1] 张学兰[1] 姜秋[1] 李慧芬[1] 王集会[1] 吴鹏[1] 王均秀 宋梦晗 

机构地区：[1]山东中医药大学,国家中医药管理局中药炮制技术传承基地,济南250355

出　　处：《中国实验方剂学杂志》2016年第16期6-9,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金面上项目(81373968)

摘　　要：目的：比较橄榄苦苷与其苷元羟基酪醇的大鼠在体胃肠吸收差异,分析橄榄苦苷在大鼠体内的代谢产物,为女贞子酒蒸增效机制研究提供科学依据。方法：采用大鼠在体胃肠灌流模型,以HPLC测定给药后不同时间点大鼠胃与小肠灌流流出液中橄榄苦苷和羟基酪醇的含量,流动相乙腈（A）-0.1%甲酸水溶液（B）梯度洗脱（0~10 min,10%~20%A;10~25min,20%~35%A）,检测波长240 nm。计算二者的吸收速率常数和累计吸收率;通过已知橄榄苦苷、羟基酪醇对照品定位,分析橄榄苦苷在大鼠胃和小肠中的代谢产物。结果：羟基酪醇在大鼠胃和小肠中的吸收速率常数和累计吸收率均显著高于橄榄苦苷;在橄榄苦苷的大鼠胃和小肠代谢物中均检出羟基酪醇。结论：羟基酪醇较橄榄苦苷更利于机体吸收,橄榄苦苷在大鼠胃和小肠中均可代谢为羟基酪醇。Objective： To compare difference between gastrointestinal absorption of oleuropein and its aglycone （hydroxytyrosol） in rats, analyze metabolites of oleuropein in rats, and provide a scientific basis for research on synergistic mechanism of Ligustri Lucidi Fructus steamed with wine. Method： Gastrointestinal perfusion model of rats was used, contents of oleuropein and hydroxytyrosol in the gastrointestinal perfusion effluent of rats were detected by HPLC after administrating at different time points, absorption rate constant （Ka ） and cumulative absorption rate were calculated. The mobile phase was acetonitrile and 0.1% formic acid aqueous solution for gradient elution, detection wavelength was set at 240 nm. By positioning of oleuropein and hydroxytyrosol reference, metabolites of oleuropein in rat＇s stomach and small intestine were determined. Result： Cumulative absorption rate in 4 h and Ka of hydroxytyrosol in rat stomach and small intestine were significantly higher than oleuropein. Hydroxytyrosol can be detected in rat stomach and intestine metabolites after pouring into oleuropein. Conclusion： Compared with oleuropein, hydroxytyrosol is more conducive to he absorbed by the body. Oleuropein can be metabolized into hydroxytyrosol both in rat stomach and small intestine.

关 键 词：橄榄苦苷 羟基酪醇 在体胃肠吸收 酚红 女贞子 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]