4种重楼不同部位4种重楼皂苷含量的分析  

Analysis of Content of Polyphyllin in Different Fractions of Four Varieties of Paridis Rhizoma

作　　者：刘佳[1] 杨亚利[1] 张鹏[1] 宋发军[1] 孟艳艳[1] 

机构地区：[1]中南民族大学生命科学学院生物技术国家民委重点试验室,武陵山区特色资源植物种质保护与利用湖北省重点试验室,武汉430074

出　　处：《中国实验方剂学杂志》2016年第16期44-48,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(31370118);中南民族大学基本科研业务费专项资金项目(CZW15019);校企合作项目(HZY14024);中南民族大学大学生创新创业资助项目(GXX14205;GCX15006;SCX15004)

摘　　要：目的:研究评价不同品种重楼的不同组织部位的药用价值。方法:分别提取华重楼、七叶一枝花、球药隔重楼和狭叶重楼的地下根茎、地上茎、叶、种子、果皮和外种皮的重楼皂苷,采用HPLC检测其重楼皂苷Ⅰ,Ⅱ,Ⅵ,Ⅶ含量。结果:4种重楼的地下根茎中重楼皂苷总含量均高于地上部分,其中,七叶一枝花的地下根茎的重楼皂苷总含量最高,为26.34 mg·g^(-1);对于地上不同组织部位而言,七叶一枝花果皮的重楼皂苷Ⅰ含量、种子的重楼皂苷Ⅵ含量、外种皮的重楼皂苷Ⅶ含量分别为10.18,0.84,8.61 mg·g^(-1);球药隔重楼种子的重楼皂苷Ⅱ含量最高,为1.1 mg·g^(-1)。结论:七叶一枝花地下根茎以及整株材料的重楼皂苷总含量最高,可作为后期人工栽培的优良品种;重楼地上茎、果皮和外种皮中的重楼皂苷Ⅰ含量与其地下根茎的含量具正相关性,可通过检测重楼地上部分的重楼皂苷Ⅰ含量初步评价其地下根茎的药用价值。Objective： This study evaluated the medicinal value of different tissue fractions of different varieties of Paridis Rhizoma. Method： Polyphyllin was extracted respectively from underground rhizomes, stems, leaves, seeds, peels and testa of Paris polyphylla var. chinensis, P. polyphylla,P. fargesii var. fargesii and P. Polyphylla var. stenophylla. The content of polyphyllin Ⅰ,Ⅱ, Ⅵ and Ⅶ were detected using HPLC method. Result： Total polyphyllin content in the underground rhizomes of the four varieties of Paridis Rhizoma was higher than that in their aboveground fractions; specifically, the total polyphyllin content in underground rhizomes of P. polyphylla was the highest, which was 26.34 mg·g^-1. In terms of different aboveground fractions, polyphyllin Ⅰ content in the peels, polyphyllin Ⅵ content in the seeds and polyphyllin Ⅶ content in the testa of P. polyphylla were the highest, which were 10.18, 0.84, 8.61 mg·g^-1, respectively. Polyphyllin Ⅱ content in the seeds of P. fargesii var. fargesii was the highest, which was 1.1 mg·g^-1. Conclusion： Due to the highest total polyphyllin content in either underground rhizomes and the whole plant of P. polyphylla among the four varieties of Paridis Rhizoma, P. polyphylla could be used as a good variety for future artificial cultivation. Polyphyllin Ⅰ content in the stems, peels and testa of Paridis Rhizoma presented positive correlations with the polyphyllin Ⅰ content in the underground rhizomes, suggesting that the medicinal value of the underground rhizomes could be preliminarily evaluated through detection of polyphyllin Ⅰ content in the aboveground fractions.

关 键 词：重楼 不同组织部位 重楼皂苷 

分 类 号：R282.2[医药卫生—中药学]