槐耳/板蓝根双向发酵体系物质基础变化  

Changes in Chemical Component Content of Fungal Substance Trametes robiniophila / Isatidis Radix Co-fermentation System

作　　者：张文意[1] 张婧[1] 高鹏飞[1] 史新元[1] 

机构地区：[1]北京中医药大学,北京100102

出　　处：《中国实验方剂学杂志》2016年第16期66-70,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：北京市科委科技新星计划交叉学科项目(xxhz201210)

摘　　要：目的:分析槐耳/板蓝根双向发酵体系的物质基础变化规律。方法:分别采用三氯甲烷和水提取不同发酵时间菌质中的脂溶性和水溶性成分,并通过UPLC-MS对提取物进行成分分析,指认发酵过程中变化明显的色谱峰,确定其分子结构。结果:槐耳/板蓝根菌质的脂溶性部分分离出11个色谱峰,经质谱确认出其中的8个化合物,分别为3-甲醛吲哚、次黄嘌呤,2,4-喹唑啉二酮,丁香酸,(E)-3-(3’,5’-二)甲氧基-4’-(羟基苯亚甲基)-2-吲哚酮,2,5-二羟基吲哚,鸟嘌呤,靛红;水溶性部分分离出8个色谱峰,经质谱确认出其中5个化合物,分别为精氨酸、甘草素、腺嘌呤、表告依春、色胺酮。结论:分离鉴定出的13种化合物具有多方面药效学作用,为后续的药效学研究提供了理论基础。Objective： To study the changes in chemical component content in fungal substance of Trametes robiniophila/Isatidis Radix co-fermentation system. Method： Chloroform and water were used respectively to extract fat-soluble and water-soluble components in fungal substance at the different fermentation time. The fat-soluble and water-soluble fractions of the co-fermentation system were analyzed by UPLC-MS, and the obviously changed chromatographic peak in the fermentation process was identified to determine the molecular structure. Result： There were 11 chromatographic peaks isolated in the fat-soluble part of Trametes robiniophila/Isatidis Radix fungal substance, and eight compounds were identified by mass spectrometry. They were 3-formaldehyde indole, hypoxanthine, 2,4-quinazoline diketone, clove acid, （E）-3-（3＇,5＇-2） methoxy-4＇-（hydroxy benzal）-2-indole ketone, 2, 5-2 hydroxy indole, guanine, and indigo red, respectively. In the water-soluble part, eight chromatographic peaks were isolated and identified by mass spectrometry. The five compounds were arginine, licorice essence, adenine, epigoitrin, and lubricious amine ketone, respectively. Conclusion： The 13 isolated compounds with various pharmacodynamics effects provide a theoretical basis for follow-up pharmacodynamics studies.

关 键 词：液质联用 槐耳 板蓝根 双向发酵 物质基础变化 

分 类 号：R284.1[医药卫生—中药学]