凌霄花总黄酮对脑缺血再灌注损伤大鼠的保护作用  

Protective Effects of Campsis Flos Total Flavonoids( CFTF) on Cerebral Ischemia Reperfusion Injury in Rats

作　　者：方晓艳[1] 吴宿慧[1] 王琳琳[1] 郑雁[1] 苗明三[1] 

机构地区：[1]河南中医药大学,郑州450046

出　　处：《中国实验方剂学杂志》2016年第16期109-113,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家教育部科学技术重点项目(211104);河南中医学院博士基金项目(BSJJ2010-01)

摘　　要：目的:研究凌霄花总黄酮对脑缺血再灌注损伤大鼠的保护作用,并探讨其作用机制。方法:SPF级Wistar大鼠60只,随机分为假手术组,模型组,阳性药组(阿司匹林片,50 mg·kg^(-1)),凌霄花总黄酮高、低剂量组(200,100 mg·kg^(-1)),除假手术组外,建立短暂性大脑中动脉阻塞大鼠模型(t MCAO),采用神经缺损评分(NDS)法评价行为学的改变、苏木素-伊红(HE)染色法评价脑组织病理形态的改变、酶联免疫吸附测定(ELISA)检测血清中神经元特异性烯醇化酶(NSE)的水平及脑组织中细胞因子白细胞介素-10(IL-10),转化生长因子-β_1(TGF-β_1)的水平,探讨凌霄花总黄酮对脑缺血再灌注损伤的保护作用。结果:与假手术组比较,模型组大鼠神经缺损评分明显升高(P<0.01),脑组织病理损伤严重,血清NSE水平明显升高(P<0.01),脑组织中IL-10,TGF-β_1水平明显降低(P<0.01);与模型组比较,凌霄花总黄酮高剂量组可明显改善模型大鼠的神经缺损症状,降低神经缺损评分(P<0.05);凌霄花总黄酮高、低剂量组和阿司匹林组能明显减轻脑组织病理损伤程度(P<0.01,P<0.05),降低血清中NSE水平(P<0.05),升高脑组织中IL-10,TGF-β_1水平(P<0.01,P<0.05)。结论:凌霄花总黄酮对短暂性脑缺血再灌注大鼠有一定的保护作用,可能与降低NSE水平,升高抗炎细胞因子IL-10,TGF-β_1的水平,减轻炎症损伤有关。Objective： To observe the protective effects of total flavonoids from Campsis Flos （CFTF） on cerebral ischemia reperfusion injury in rats. Method： Sixty SPF grade Wistar rats were randomly divided into sham operation group, model group, positive drug group （aspirin, 50 mg·kg^-1）, CFTF high dose and low dose groups （200, 100 mg·kg^-1）. Models of transient middle cerebral artery occlusion （tMCAO） were established in all the rats except those in sham operation group. The behavioral injury was detected by neurologic deficit scores （NDS）; the cerebral histopathology was detected by HE staining; and the levels of neuron specific enolase （NSE） in serum, interleukin（IL）-10, and TGF-β1 in brain homogenate were detected by ELISA, to discuss the protective effect of CFTF on cerebral ischemia reperfusion injury. Result： As compared with sham operation group, the neurologic deficit scores were significantly higher in rats of model group （P〈0.01）, with serious cerebral histopathology injuries; and the level of NSE in serum was significantly increased （P〈0.01）; the levels of IL-10, and TGF-β1 in brain homogenate were decreased dramatically （P〈0.01）. As compared with the model group,the neurologic deficit symptoms were significantly improved in CFTF high dose group （200 mg·kg^-1）, with decreased neurologic deficit scores （P〈0.05）; cerebral histopathology injury was significantly decreased（P〈0.01, P〈0.05）, NSE level in serum was decreased （P〈0.05）, and levels of IL-10, TGF-β1 in brain tissues were increased （P〈0.01, P〈0.05） in CFTF high dose and low dose groups （200, 100 mg·kg^-1） and aspirin group. Conclusion： CFTF had the protective effects on tMCAO rats by decreasing the level of NSE, increasing the levels of anti-inflammation cytokines （IL-10 and TGF-β1）, and attenuating the inflammation injury.

关 键 词：凌霄花总黄酮 脑缺血再灌注 神经缺损评分 神经元特异性烯醇化酶 抗炎细胞因子 

分 类 号：R285.5[医药卫生—中药学]