正交设计法研究羟基脲和米非司酮的优选配比制作大鼠流产模型  

Orthogonal Design Method to Study Optimal Ratio of Hydroxyurea and Mifepristone in Miscarriage Models of Rats

作　　者：郜洁[1] 巫海旺 杜鑫[1] 林娜[2] 罗颂平[1] 

机构地区：[1]广州中医药大学第一临床医学院,广州510405 [2]中国中医科学院中药研究所,北京100700

出　　处：《中国实验方剂学杂志》2016年第16期114-118,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金青年项目(81001533);中国博士后科学基金项目(20110490557);2015年中医药强省专项基金医院中药制剂建设项目(粤中医办函[2015]102号)

摘　　要：目的：筛选制作肾虚-黄体抑制流产大鼠流产模型药物羟基脲和米非司酮的优选配比。方法：将100只雌性SD大鼠与50只雄性SD大鼠合笼,按照正交表L9（3^4）设计,共有9个受试样品,再平行增设阴性组（生理盐水）,共10个组。妊娠第1天开始除阴性组灌服蒸馏水外各组动物灌服羟基脲按照正交设计表的剂量进行造模,配种后第1,4,9天测量动物的体重,第10天除外各组动物早上分别按照正交设计表的剂量加灌米非司酮。分别观察大鼠一般情况,测定体重变化、胚胎直径、计算胚胎流产率。结果：模型1~4,6~8组在怀孕第11天的胚胎直径与阴性组比较显著降低（P〈0.05）。模型1~9组在怀孕第11天的流产率与阴性组比较显著增高（P〈0.05）。在流产率和胚胎直径方面,米非司酮贡献度大于羟基脲。结论：以直观指标流产率和胚胎直径为衡量评价指标,初步筛选了模型2,3,6组的米非司酮与羟基脲的优选配比。Objective： To screen optimal ratio of hydroxyurea and mifepristone of kidney deficiency and luteum inhibition models of miscarriage rats. Method： After cohabitating 100 female SD rats and 50 male rats,there were 9 subjects according to the orthogonal table L9 （3^4） design. In addition to this, control group （normal saline） was added. Therefore, there were 10 groups altogether. Beginning with first day of pregnancy,the control group was administered with distilled water while other groups were administered with hydroxyurea according to orthogonal design table. All weights of the rats were measured in the 1st,4th,9th day after mating. During the 10th day,all groups were administered with mifepristone according to orthogonal design table except the control group. Then observed the general conditions of the rats,measured the weights,embryo diameter,calculated the miscarriage rate. Result： The embryo diameter of model group 1-4,6-8 decreased significantly （P〈0.05） comparing with control group during the 11th day of pregnancy. The miscarriage rate of model group 1-9 increased significantly （P〈0.05） comparing with control group during the 11th day of pregnancy. Therefore,mifepristone is more of contribution than hydroxyurea in miscarriage rate and embryo diameter. Conclusion： The miscarriage rate and embryo diameter are as the core indicators to evaluate pregnant rats and to screen the optimal ratio of hydroxyurea and mifepristone of model group 2,3,6.

关 键 词：流产 动物模型 正交设计 羟基脲 米非司酮 

分 类 号：R285.5[医药卫生—中药学]