人参多糖注射液对肝细胞癌的增殖抑制作用及机制探讨  

Discussion on Effects and Mechanism of Ginseng Polysaccharide Injection on Proliferation Inhibition of Hepatoma Cells

作　　者：高恒宇[1] 郭林娜[1] 刘丹[1] 

机构地区：[1]齐齐哈尔医学院,黑龙江齐齐哈尔161006

出　　处：《中国实验方剂学杂志》2016年第16期134-138,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：黑龙江省教育厅科学技术研究面上项目(12531831)

摘　　要：目的:观察人参多糖注射液对人肝癌细胞BEL-7402和HpeG2增殖抑制作用,并探讨其机制。方法:将不同浓度的人参多糖注射液与人肝癌细胞BEL-7402和HpeG2作用,采用cell counting kit-8(CCK8)检测细胞的增殖抑制,通过倒置显微镜观察用药前后细胞形态、数量变化,透射电镜观察细胞坏死、凋亡等形态学变化,蛋白免疫印迹法(Western blot)检测肿瘤坏死因子受体1(TNFR1)蛋白表达情况。结果:人参多糖注射液对人肝癌细胞BEL-7402和HpeG2的增殖有明显抑制作用(P<0.05),并且与浓度和时间呈正相关。60,80,100 g·L^(-1)人参多糖注射液作用于人肝癌细胞BEL-7402和HpeG2 24,48,72h后,可显著抑制其增殖(P<0.01),40 g·L^(-1)人参多糖注射液作用于人肝癌细胞BEL-7402和HpeG2 24,48,72 h后可明显抑制其增殖(P<0.05)。人参多糖注射液作用人肝癌细胞BEL-7402和HpeG2 48 h后,细胞出现细胞核边集、凋亡小体等凋亡形态变化,72 h后部分细胞出现空泡样变等形态学变化。与空白组比较,40,60,80 g·L-1人参多糖注射液组作用人肝癌细胞BEL-7402和HpeG2后,TNFR1蛋白相对表达量明显升高(P<0.05,P<0.01)。结论:人参多糖注射液对人肝癌细胞BEL-7402和HpeG2的增殖有明显的抑制作用;诱导细胞凋亡可能是抑制作用的机制。Objective： To investigate the effects and mechanism of ginseng polysaccharide injection on the proliferative inhibition of hepatoma cell line BEL-7402 and HpeG2. Method： The hepatoma cell line BEL-7402 and HpeG2 were treated with different concentrations of ginseng polysaccharide injection. Cell counting kit-8 （CCK8） was used to detect the proliferative inhibition of cells. Inverted microscope was used to observe the changes both in quantity and morphology of the cells before and after the treatment. Transmission electron microscope （TEM） was used to observe the morphological changes such as apoptosis and necrosis. Western blot was used to detect the expression levels of tumor necrosis factor receptor 1 （TNFR1）. Result： Ginseng polysaccharide injection could effectively inhibit the proliferation of BEL-7402 and HpeG2 cells （P〈0.05）, which was positively correlated with concentration and time. It could significantly inhibit the proliferation of BEL-7402 and HpeG2 cells after 24, 48, 72 h treatment with the concentrations of 60, 80 and 100 g·L^-1 ginseng polysaccharide injection （P〈0.01）. It could also inhibit the proliferation of BEL-7402 and HpeG2 cells after 24, 48, 72 h treatment with the concentration of 40 g·L^-1 （P〈0.05）. The results of TEM showed that ginseng polysaccharide injection resulted in morphological changes of BEL-7402 and HpeG2 such as nuclear edge set and apoptotic bodies after 48 hours treatment, and vacuoles appeared in part cells after 72 hours treatment. As compared with the blank group, after BEL-7402 and HpeG2 cells were treated with 40, 60, 80 g·L^-1 ginseng polysaccharide injection, the relative expressions of TNFR1 protein were significantly increased （P〈0.05, P〈0.01）. Conclusion： Ginseng polysaccharide injection can effectively inhibit the proliferation of hepatoma cell line BEL-7402 and HpeG2, and the mechanism may be associated with induction of apoptosis.

关 键 词：肝细胞癌 人参多糖注射液 BEL-7402细胞 HpeG2细胞 凋亡 肿瘤坏死因子受体1 

分 类 号：R285.5[医药卫生—中药学]