槲皮素抑制人前列腺癌PC-3细胞增殖及与Wnt/β-catenin信号通路的机制  

Anti-proliferation Effect of Quereetin on Human Prostate Cancer PC-3 Cells and Wnt/β-catenin Signaling Pathway

作　　者：张智慧[1] 白吉祥[1] 

机构地区：[1]牡丹江医学院红旗医院,黑龙江牡丹江157011

出　　处：《中国实验方剂学杂志》2016年第16期144-148,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：黑龙江省卫生计生委科研课题项目(2014-203)

摘　　要：目的:观察槲皮素对人前列腺癌PC-3细胞增殖和Wnt/β-连环蛋白(β-catenin)信号通路的调控作用,探讨其抗前列腺癌的可能机制。方法:体外培养PC-3细胞,以不同终浓度槲皮素进行处理,设空白组。采用噻唑蓝比色法(MTT)观察槲皮素对PC-3细胞增殖抑制作用,计算半数抑制浓度(IC50);采用流式细胞仪检测槲皮素对PC-3细胞凋亡的影响;采用免疫印迹法(Western blot)和逆转录-PCR(RT-PCR)分析检测槲皮素对β-catenin和下游靶基因细胞周期素D1(Cyclin D1),原癌基因(c-Myc)蛋白表达和转录水平;采用免疫荧光分析检测槲皮素对β-catenin表达的影响;利用荧光酶报告基因分析检测槲皮素对Wnt/β-catenin信号通路的影响。结果:与空白组比较,槲皮素对PC-3细胞增殖具有明显抑制作用(P<0.05),并诱导PC-3细胞凋亡(P<0.05)。与空白组比较,槲皮素可显著抑制PC-3细胞β-catenin的蛋白表达和转录活性,从而阻断Wnt/β-catenin信号通路的传导,抑制下游靶基因的表达水平(P<0.05,P<0.01)。结论:槲皮素可显著抑制PC-3细胞增殖,其机制可能与抑制Wnt/β-catenin信号通路有关。Objective： To observe the anti-proliferation effect of quereetin on human prostate cancer PC-3 cells and Wnt/β-catenin signaling pathways, and explore its possible mechanisms of anti-prostate cancer. Method： PC-3 cells were cultured in vitro and treated with capsaicin at various concentrations. The cells without quereetin treatment were used as blank group. Methyl thiazolyl tetrazolium （MTT） assay was used to observe anti-proliferation effect of quereetin on PC-3 cells, and 50%inhibitory concentration （IC50） was calculated. Apoptosis of PC-3 cells was observed by flow cytometry, the protein expressions and transcriptional levels of β-catenin and its downstream target genes （Cyclin D1 and c-Myc） were analyzed by Western blot and reverse transcription-PCR（RT-PCR）, immunofluorometric assay was used to detect the effect of quereetin on β-catenin expressions, luciferase reporter assay was performed to detect the effect of quereetin on Wnt/β-catenin signaling pathways in PC-3 cells. Result： As compared with the blank group, quereetin significantly inhibited proliferation of PC-3 cells （P〈0.05） and induced their apotosis （P〈0.05）. Quereetin also significantly reduced protein expressions and transcriptional levels of β-catenin, suppressed the Wnt/β-catenin signaling pathway and down-regulated the expressions of downstream target genes （P〈0.05, P〈0.01）. Conclusion： Quercetin could significantly inhibit the proliferation of PC-3 cells and its mechanism may be related to the inhibition of Wnt/β-catenin signaling pathways.

关 键 词：前列腺癌 槲皮素 Wnt/β-连环蛋白信号通路 细胞周期素D1 原癌基因 

分 类 号：R285.5[医药卫生—中药学]