抗真菌抗生素CA1189产生菌SIIA-A1626的鉴定  

Indentificaiton of strain SIIA-A1626 producing antifungal antibiotic CA1189

作　　者：郭义东[1] 雨田[2] 刘超兰[1] 

机构地区：[1]抗生素研究与再评价四川省重点实验室,四川抗菌素工业研究所,成都大学,成都610052 [2]基础医学与护理学院,成都大学,成都610106

出　　处：《中国抗生素杂志》2016年第8期590-593,605,共5页Chinese Journal of Antibiotics

基　　金：四川省医药微生物共享服务平台建设项目

摘　　要：目的抗真菌抗生素CA1189产生菌SIIA-A1626的分类鉴定。方法采用形态观察、生理生化特征鉴定、16S r RNA基因序列对比及系统发育学分析等相结合的多相分类研究方法来确定该菌株的分类学地位。结果与结论菌株SIIA-A1626在高氏1号培养基和ISP系列培养基上的形态特点和培养特征都呈现出链霉菌属菌株的形态学特征;菌株SIIA-A1626的16S r RNA基因与菌株Streptomyces violascens NBRC 12920T相似性最高,达到99.18%;在基于16S r RNA基因序列分析构建的系统进化树上,菌株SIIA-A1626在链霉菌属簇内形成独立分支。综合以上实验结果,菌株SIIA-A1626可能是链霉菌属的一个新种。Objecive To identify strain SIIA-A1626, producing antifungal antibiotic CA1189. Methods Polyphasic taxonomy based on morphology, physiological properties and 16S rRNA gene phylogenetic analysis were adopted to determine the taxonomic status of strain SIIA-A1626. Results and Conclusion Morphological characteristics and culture features of strain SIIA-A1626 on Gause 1 medium and ISP series media were the same as the morphological characteristics of genus Streptomyces. Phylogenetic analysis based on 16S rRNA gene sequencing revealed that the strain formed a monophyletic clade with the closely related type strains of Streptomyces and was most closely related to Streptomyces violascens NBRC 12920T （99.18%）. On the basis of the above results, Strain SIIA-A 1626 could be preliminarily considered to represent a novel species of the genus Streptomyces.

关 键 词：链霉菌 多相分类 

分 类 号：R978.5[医药卫生—药品] Q93[医药卫生—药学]