T淋巴瘤侵袭转移诱导因子1mRNA定量检测与结直肠癌淋巴结微转移及预后的关系  

Correlation between quantitative expression of T lymphoma invasion and metastasis inducing factor 1 mRNA and lymph node micrometastasis and prognosis in patients with colorectal cancer

作　　者：刘少平[1] 张海 聂磊[2] 胡亚华[1] 张险峰[1] 朱燕莉[1] 

机构地区：[1]湖北理工学院附属黄石市中心医院消化科,435000 [2]湖北省肿瘤医院腹部外科

出　　处：《中华消化杂志》2016年第8期526-531,共6页Chinese Journal of Digestion

基　　金：湖北省自然科学基金（2010CDB07401）

摘　　要：目的探讨结直肠癌组织中T淋巴瘤侵袭转移诱导因子1（Tiam1）mRNA表达量的变化与结直肠癌淋巴结微转移和淋巴结阴性结直肠癌患者预后的关系。方法纳入2008年6月至2010年8月的63例淋巴结转移阴性的结直肠癌患者、20例淋巴结转移阳性的结直肠癌患者和25例结直肠良性病变患者，采用实时荧光定量PCR检测结直肠癌组织和结直肠良性病变组织中Tiam1 mRNA的相对表达量，同时检测前哨淋巴结组织中角蛋白20的表达以评价淋巴结微转移，随访淋巴结阴性结直肠癌患者术后5年预后状况，分析癌组织中Tiam1 mRNA表达量与淋巴结微转移和预后的关系。统计学分析分别采用t检验、单因素分析、多因素分析和Log-rank法。结果63例淋巴结阴性的结直肠癌患者中，33例有淋巴结微转移。淋巴结阳性结直肠癌组的Tiam1 mRNA相对表达量为9.84±2.36，高于淋巴结阴性结直肠癌组的5.15±3.58，差异有统计学意义（t＝5.479，P〈0.01）；这两组的Tiam1 mRNA相对表达量均高于结直肠良性病变组的0.30±0.21，差异均有统计学意义（t＝20.169、6.745，P均〈0.01）。淋巴结微转移阳性组的Tiam1 mRNA相对表达量为6.30±1.95，高于淋巴结微转移阴性组的3.88±1.63，差异有统计学意义（t＝5.330，P〈0.01）。淋巴结阴性结直肠癌患者肿瘤分化程度越低，浸润深度越深，术后生存期越短，术后有复发转移，则癌组织中Tiam1 mRNA相对表达量越高（t＝2.536、3.112、3.213、2.676，P均〈0.05）。单因素分析显示，癌组织中Tiam1 mRNA表达与淋巴结微转移相关（χ^2 ＝11.878，P＝0.001）；多因素分析显示，其是结直肠癌淋巴结微转移独立的危险因素（相对危险度为9.782）。Tiam1 mRNA高表达组术后5年累积生存率为75.8%，低于低表达组的97.1%，差异有统计学意义（χ^2 ＝4.575，P〈0.05）。结论Tiam1可能参与结直肠癌淋巴结转移的调控，�ObjectiveTo investigate the relationship between the changes of expression of T lymphoma invasion and metastasis inducing factor 1（Tiam1） mRNA and lymph node micrometastasis and prognosis in patients with node-negative colorectal cancer（CRC）. MethodsFrom June 2008 to August 2010, a total of 63 patients with lymph node-negative CRC, 20 patients with lymph node-positive CRC and 25 patients with colorectal benign lesions were enrolled. The relative quantity expression （RQ） of Tiam1 mRNA in CRC cancer tissues and colorectal benign lesions were detected by real-time quantitative polymerase chain reaction （PCR）. The lymph node micrometastasis was determined by cytokeratin 20 detection in lymph nodes tissues. The five-year postoperative prognosis was evaluated by followed-up in the patients with lymph node-negative CRC. The correlation between the expression of Tiam1 mRNA and lymph node micrometastasis and prognosis was analyzed. The t-test, univariate analysis, multivariate analysis and Log-rank test were performed for statistical analysis.ResultsLymphnode micrometastasis occurred in 33 from 63 patients with lymph node-negative CRC. The RQ of Tiam1 mRNA in the patients with lymph node-positive CRC was 9.84±2.36, which was higher than that of patients with lymph node-negative CRC （5.15±3.58）, and the difference was statistically significant （t=5.479, P〈0.01）. The RQ of Tiam1 mRNA of these two groups were both higher than that of colorectal benign lesion （0.30±0.21）, and the difference was statistically significant（t=20.169, 6.745; both P〈0.01）. The RQ of Tiam1 mRNA of positive lymph node micrometastasis group was 6.30±1.95, which was higher than that of negative lymph node micrometastasis group （3.88±1.63）, and the difference was statistically significant （t=5.330, P〈0.01）. In the patients with lymph node-negative CRC, the lower degree of tumor difference, the deeper invasion, the shorter survival time after surgery and postoperative recurrence and metastasis were rela

关 键 词：Tiaml MRNA 结直肠癌 淋巴结微转移 预后 

分 类 号：R735.34[医药卫生—肿瘤]