3种秋眠类型苜蓿对不同CO_2浓度的生理响应  

Effect of different CO_2 concentrations on physiological responses of fall-dormant alfalfa

作　　者：翟晓朦 王铁梅[2] 关潇[1] 张晓波[3] 

机构地区：[1]中国环境科学研究院环境基准与风险评估国家重点实验室,北京100012 [2]北京林业大学林学院,北京100083 [3]海南大学旅游学院,海南海口570228

出　　处：《草业科学》2016年第8期1550-1559,共10页Pratacultural Science

基　　金：2012年环保公益项目--气候变化下保护优先区脆弱性评估与保护对策研究(201209031)

摘　　要：利用开顶式气室(open-top chamber,OTC)研究了3种秋眠类型苜蓿(Medicago sativa)(秋眠型苜蓿Maverick、半秋眠型苜蓿ABI700和极非秋眠型苜蓿UC-1465)在700、550和350μmol·mol-1(CK)3种CO_2浓度处理下整个生育期的生理变化。结果表明,整个生育期CO_2浓度越高,苜蓿叶绿素和可溶性糖含量越高。700和550μmol·mol-1处理下,3种秋眠类型的苜蓿平均叶绿素含量较对照平均分别提高了约11.49%和6.00%,可溶性糖含量平均分别提高了约52.01%和38.82%。CO_2浓度升高能够降低苜蓿的丙二醛含量,700和550μmol·mol-1处理下,丙二醛含量较照平均分别下降了约18.20%和17.93%。盛花期时,CO_2浓度升高对脯氨酸合成量有显著的正效应(P<0.05),其余时间呈抑制状态。综合来说,CO_2浓度升高在初花期时对苜蓿各生理指标均有显著影响(P<0.05)。高CO_2浓度处理下,极非秋眠型苜蓿UC-1465的叶绿素累计合成量高于秋眠型苜蓿Maverick和半秋眠型苜蓿ABI700,而高CO_2浓度大大促进了秋眠型苜蓿Maverick、半秋眠型苜蓿Maverick合成可溶性糖、降低丙二醛,有利于增强其缓解胁迫以及渗透调节能力。To study the physiological changes process of three types of fall dormancy alfalfa under three CO2 concentration （700 μmol· mol^-1, 550 μmol· mol^-1 and control 350 μmol· mol^-1） in open-top chambers during the whole growth period. The results showed that： the CO2 concentration higher, the chlorophyll content and the content of soluble sugar higher of the whole growth period. The chlorophyll content was increased by an average of about 11.49%, 6.00%; soluble sugar content was increased by an average of 52.01%, 38.82% with 700 and 550 μmol· mol^-1treatment. The MDA content decreased by an average of approximately 18.20%, 17.93% with 700 and 550 μmol· mol^-1 treatment. The elevated CO2 concentration had a significant positive effect on the synthesis of proline content at flowering （P〈0.05） and the rest of the time was inhibited. In summary, CO2 concentration had a significantly effect on physiological indices of alfalfa at the early flowering （P〈0.05）. The chlorophyll synthesis cumulative amount of UC-1465 （non-fall dormancy alfalfa） higher than Maverick （fall dormancy alfalfa） and ABI700 （semi fall dormancy alfalfa） under high CO2 concentrations. And high CO2 concentration is more favorable for Maverick （fall dormancy alfalfa）, ABI700 （semi fall dormancy alfalfa） to accumulate soluble sugar and reduce MDA content, it is very likely to enhance the capacity of ease stress and osmotic adjustment.

关 键 词：CO2浓度 秋眠型苜蓿 生理指标 叶绿素 

分 类 号：S541.9[农业科学—作物学]