干旱胁迫对转拟南芥AtP5CS1基因羽衣甘蓝光合特性的影响  

Effects of drought stress on photosynthetic characteristics of kale genetically modified by AtP5CS1 gene of Arabidopsis

作　　者：李大红[1] 李伟[1] 邵海阔 

机构地区：[1]黄淮学院生物工程系 [2]黄淮学院园林中心,驻马店463000

出　　处：《上海农业学报》2016年第4期6-10,共5页Acta Agriculturae Shanghai

摘　　要：以转拟南芥AtP5CS1基因羽衣甘蓝和野生型(对照)为试验材料,在水分胁迫0 d、2 d、4 d、6 d和复水2 d、4 d条件下,研究水分胁迫和复水后叶片的气体交换、叶绿素荧光参数、叶绿素含量的变化特征。结果表明:随着水分胁迫天数的增加,转基因羽衣甘蓝的叶绿素含量、净光合速率(Pn)、气孔导度(Gs)、胞间CO_2浓度(Ci)、蒸腾速率(Tr)、水分利用效率(WUE)、Fv/Fm等指标的下降幅度均小于对照,气孔限制值(Ls)和非光化学猝灭系数(NPQ)上升的幅度小于对照,说明转基因羽衣甘蓝的光系统在水分胁迫时受到的伤害程度要小于对照;复水后转基因羽衣甘蓝的光合参数、叶绿素荧光参数、叶绿素含量等迅速恢复,而对照的光合参数及其他参数恢复缓慢。在水分胁迫条件下,转基因羽衣甘蓝能保持良好的光合特性,复水后具有良好的生理补偿效应,是适应干旱环境的重要生理因素。Pot experiments with both kale genetically modified by AtP5 CSl gene of Arabidopsis and wild kale（CK） were carried out,and their leaf variations in gas exchange and chlorophyll content and fluorescence parameters were studied under water stress for 0 d,2 d,4 d and 6 d and rehydration for 2 d and 4 d.The results showed that with water stress duration increasing,the genetically modified kale＇ s indicators such as chlorophyll content,net photosynthetic rate（Pn）,stomatal conductance（Gs）,intercellular CO_2 concentration（Ci）,transpiration rate（TV）,water use efficiency（WUE） and Fv/Fm（maximal efficiency of PS Ⅱ photochemistry）decreased less than the CK＇ s,and the genetically modified kale＇ s stomatal limitation value（Ls） and nonphotochemical quenching coefficient（NPQ） increased less than the CK＇ syndicating that the damage by water stress to the genetically modified kale was less than that to CK;After watering,the genetically modified kale＇ s photosynthetic parameters,chlorophyll fluorescence parameters and chlorophyll content recovered rapidly,whereas the CK＇ s recovered slowly.Under water stress the genetically modified kale could maintain good photosynthetic characteristics and have a good physiological compensating action after watering,being an important physiological factor for adaptation to arid environments.

关 键 词：羽衣甘蓝 转基因 抗旱性 光合特性 对比试验 

分 类 号：S635.9[农业科学—蔬菜学]