几种理化因子对黄芩悬浮细胞生长及黄芩苷含量的影响  

Effects of some physical-chemical factors on growth of suspension cells and contents of baicalin from Scutellaria baicalensis

作　　者：毕宇[1] 张东向[1] 刘丽杰[1] 任艳波[1] 

机构地区：[1]齐齐哈尔大学生命科学与农林学院,黑龙江齐齐哈尔161006

出　　处：《高师理科学刊》2016年第8期42-46,共5页Journal of Science of Teachers＇College and University

基　　金：黑龙江省教育厅科研资助项目(12521609)

摘　　要：以黄芩悬浮细胞为试材,研究不同接种量、蔗糖质量浓度以及6-BA和NAA不同配比对黄芩悬浮细胞培养过程中干/鲜质量和黄芩苷含量变化的影响.结果表明,黄芩悬浮培养的最佳接种量为每瓶1.0 g;蔗糖浓度为3%时,最适合黄芩悬浮细胞系生长;pH值为5.8时最适合黄芩悬浮细胞系生长和代谢产物积累的培养基;0.1 mg·L^(-1) NAA与0.2 mg·L^(-1) 6-BA配比时,最适合黄芩悬浮细胞系的生长.Using the suspension cells from Scutellaria baicalensis as material,the effects of inoculum amounts,concentration of sugar and combinations of 6BA and NAA on the growth of suspension cells and contents of baicalin from Scutellaria baicalensis were studied.The results showed that the inoculum size of Scutellaria baicalensis was 1.0 g per bottle by suspension cultures,the sucrose concentration being 3%,the optimal pH value was 5.8,the combination of 0.1 mg/L NAA and 0.2 mg/L 6-BA was optimal for the growth of the suspension cell line.

关 键 词：黄芩 悬浮细胞 生物量 黄芩苷 

分 类 号：Q945[生物学—植物学]