介质膜结构空芯光纤酶基传感器的制作与优化  

Fabrication and Optimization of Enzyme Biosensors Based on Hollow Fiber with Dielectric Film

作　　者：何宇婧[1] 陈盼[1] 朱晓松[1] 汤晓黎[1] 石艺尉[1] 

机构地区：[1]复旦大学通信科学与工程系,上海200433

出　　处：《光电工程》2016年第8期18-26,共9页Opto-Electronic Engineering

基　　金：国家自然科学基金(61201062);上海市自然科学基金(15ZR1404100)资助项目

摘　　要：设计了一种基于介质膜结构的空芯光纤化学发光传感器。建立了多层膜空芯光纤非子午线几何光学传输模型,对其损耗谱和发光特性进行了仿真分析,并考虑了介质膜界面粗糙度、介质膜厚度等相关参数对化学发光及传输特性的影响。改进了溶胶-凝胶酶基敏感膜的制作工艺,提出了分离镀膜法,并对敏感膜厚度进行了实测分析。搭建了基于介质膜空芯光纤化学发光传感器的检测系统,对过氧化氢浓度进行了实际测量。结果表明,基于本传感器的系统,得到了22.8 n W/(mmol·L-1)的灵敏度以及3μmol/L的检测极限。实现了近200 h的使用寿命。且操作简单,反应快速,可小型化,可应用于环境监测,生物分析等领域。A chemiluminescence sensor is proposed based on the dielectric coated silver hollow fiber. A ray optics model is established for the multi-layer hollow fiber to analyze the loss spectrum and chemiluminescent characteristics. And parameters such as film surface roughness and thickness are taken into consideration. The fabrication techniques for coating sol-gel sensing film inside the hollow fiber are developed. A sensing system is designed and established for detecting hydrogen peroxide concentration and a high sensitivity （22.8 nW/（mmol·L^-1）） and good detection limit （3μmol/L） are achieved. This sensor has a good performance in 200 hours. It has potential applications in environmental monitoring and biological analysis fields for its simple operation, fast response, and miniaturization.

关 键 词：生物传感器 多层膜空芯光纤 化学发光 酶 溶胶-凝胶法 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]