交配后扶桑绵粉蚧雌成虫卵巢结构及其他相关结构的变化(英文)  

Structure of ovaries and changes in the reproductive components of female Phenacoccus solenopsis adults after mating

作　　者：黄芳 吕要斌 赵春玲 宋西娇 

机构地区：[1]断江省农业科学院植物保护与微生物研究所,杭州310029 [2]湖州出入境检验检疫局,浙江湖州313000 [3]浙江省农业科学院公共实验室,杭州310029

出　　处：《环境昆虫学报》2016年第4期715-722,共8页Journal of Environmental Entomology

基　　金：浙江省自然科学基金项目(LQ14C140002);国家自然科学基金项目(31270580);农业部公益性行业专项(201103026)

摘　　要：利用显微光镜和超微电镜技术观察明确了扶桑绵粉蚧雌成虫卵巢及相关结构的变化,结果表明该虫的内生殖系统含1对卵巢、输卵管、储精囊和1对附腺。每个卵巢有数以百计的端滋式卵巢小管。卵巢小管缺末端纤丝,包含滋养部分和卵黄部分。储精囊表面被丰富的肌原纤维包裹,未经交配的雌虫体内储精囊在显微镜下呈透明状圆形,电镜观察囊内只含液态物质;交配后,储精囊不再维持规则的球状,囊内出现精细胞等物质;精细胞呈典型的"9+2"结构。在初孵化的雌性成虫体内,卵巢小管内的滋养细胞部分中间为营养核,以此联接滋养细胞和卵细胞。卵黄部分包含1个卵细胞。雌性成虫只有通过交配,卵巢内的胚胎才可得以顺利发育;若未经交配,卵巢内的卵细胞将出现发达的内质网结构,标志着细胞将降解而被母体重吸收。Structure of ovaries and changes in reproductive components of female Phenacoccus solenopsis were studied using light microscopy and transmission electron microscopy. Reproductive system of female P. solenopsis was composed of a pair of ovaries, a common oviduct, a spermatheca and two pairs of accessory glands. Each ovary was composed of approximately hundreds of telotrophic ovarioles. The ovariole was devoid of terminal filaments, and was subdivided into an apical tropharium and a vitellarium. Spermatheca was surrounded by a network of myofibril. Before mating, the spermatheca was round and translucent in the microscopy observation; nothing except liquid substance was observed under TEM. Once mated, the round shape could not be supported, and the sac was full -filled with substances proposed from the males involving with sperms. Sperms of P. solenopsis had a typical characteristic ＂9 ＋ 2＂ structure. In the newly emerged female adults, the center of the tropharium was occupied by atrophic core, through which trophocytes and oocytes were connected. The vitellarium contained one oocyte. Only if the females were mated, sperms in the sac of the spermatheca, and embryogenesis in the ovarioles could be observed; otherwise, endoplasmic reticulum in the inner layer of the ovariole developed well, which was proposed to absorb the substance in the degenerated oocytes.

关 键 词：扶桑绵粉蚧 雌成虫 生殖系统 结构 

分 类 号：Q965[生物学—昆虫学] S433[农业科学—农业昆虫与害虫防治]