大蜡螟气味受体基因Gmel/Orco的克隆与序列分析  

Gene cloning and sequence analysis of( greater wax moth ) the odorant receptor gene Gmel / Orco in Galleria mellonella L.

作　　者：杨爽[1,2] 赵慧婷[3] 宋文菲[1] 孟娇[2] 杨珊珊[2] 杜亚丽[2] 潘建芳[2] 王树杰[2] 姜玉锁[2] 

机构地区：[1]云南省农业科学院蚕桑蜜蜂研究所,云南蒙自661101 [2]山西农业大学动物科技学院,太谷030801 [3]山西农业大学生命科学学院,太谷030801

出　　处：《环境昆虫学报》2016年第4期798-804,共7页Journal of Environmental Entomology

基　　金：云南省农业科学院蚕桑蜜蜂研究所青年创新基金(QC2013003)

摘　　要：为进一步研究大蜡螟嗅觉通讯分子机制和寻求新的大蜡螟防治技术,本研究克隆了大蜡螟Galleria mellonella L.的气味受体基因Gmel/Orco,并对其序列进行生物信息学分析。根据GenBank中已发表的鳞翅目昆虫非典型气味受体家族基因的氨基酸保守序列设计简并引物,采用RT-PCR方法扩增目的基因,将其克隆至T载体并测序。克隆获得大蜡螟气味受体Orco的cDNA序列,命名为Gmel/Orco(GenBank登录号:KT020861),序列分析结果显示,Gmel/Orco开放阅读框长1425 bp,编码474个氨基酸,分子量为53.36 k D,等电点为8.44,序列中有7个跨膜区,N-端在细胞膜内,C-端在细胞膜外。通过在Gen Bank中进行序列的同源性比较,该基因与已公布的鳞翅目螟蛾科、夜蛾科昆虫的非典型气味受体基因序列有较高的同源性。克隆所获得的基因属于非典型气味受体家族基因。For further study the mechanism of olfactory communication and seek new prevention technology in Galleria mellonella L.（ greater wax moth）,the olfactory receptor gene（ Gmel / Orco） was cloned,and analysed by bioinformatics analysis. According to the conserved amino acid sequences of the Lepidoptera insects olfactory receptor gene family published in Gen Bank,the degenerate primer were designed for RT-PCR. The c DNA sequence of wax moth olfactory receptor gene was cloned,and named Gmel / Orco（ Gen Bank accession number： KT020861）,the results of sequence analysis showed that the open reading frame of Gmel / Orco consisted of 1425 nucleotides,encoding 474 amino acid with a molecular weight of 53. 36 k D,an isoelectric point of 8. 44 and 7 putative transmembrane domains in the sequence,with an intracellular N-terminal and extracellular C-terminus. By comparing the homology of the sequences in Gen Bank,the sequence shared high identy with Orco orthologue chemoreceptor sequences from other previously reported insects. The result indicated that the gene belongs to atypical olfactory receptor gene family.

关 键 词：大蜡螟 气味受体 基因克隆 序列对比 

分 类 号：Q963[生物学—昆虫学] S895.2[农业科学—特种经济动物饲养]