伞裙追寄蝇不同地理种群遗传多样性的ISSR分析  

Genetic diversity of Exorista civilis from different geographical populations based on ISSR marker

作　　者：王梦圆[1,2] 韩海斌[2] 王惠萍 岳方正 丛靖宇[1] 刘爱萍[2] 

机构地区：[1]内蒙古农业大学,呼和浩特010010 [2]中国农业科学院草原研究所,呼和浩特010010 [3]锡林郭勒盟太仆寺旗草原站,内蒙古宝昌027000

出　　处：《环境昆虫学报》2016年第4期805-812,共8页Journal of Environmental Entomology

基　　金：公益性行业项目(201103002);"十二五"国家科技支撑计划项目(2012BAD13B07);农业部"948"项目(2011-G4)

摘　　要：为从分子水平探索不同地区伞裙追寄蝇种群间的内在联系,本文利用ISSR分子标记技术对8个不同地区伞裙追寄蝇自然种群进行遗传多样性、种群间分化程度以及聚类分析等研究。结果表明:筛选出11对多态性稳定的ISSR引物,对8个地区伞裙追寄蝇群体的80个个体进行PCR扩增,共获得166个重复性好且清晰可辨的ISSR条带,平均每条引物扩增出15.0909个片段,且均为多态性条带,多态信息含量(PIC)为0.8441-0.8653;香农信息指数(I)为0.1240-0.3455;Nei's遗传多样性指数(H)为0.0841-0.2285;基因分化率(Gst)为28.78%,基因流(Nm)的均值为1.5702,即遗传变异主要存在于个体之间,不同种群间基因交流处于中等水平;8个地区伞裙追寄蝇种群被划分为4个类群,种群间的遗传分化与地理距离呈正相关关系。In order to explore the genetic link of the different geographic populations of Exorista civilis at the molecular level,the molecular marker technique based on inter-simple sequence repeat（ ISSR） was used to assess the genetic diversity,population differentiation and cluster analysis in natural populations of E. civilis from eight different regions. The results showed that eleven primers with stable polymorphic were sieved from primers of ISSR and a total of 166 clear ISSR bands with high repeatable were amplified from the 80 individuals,on average,each primer amplified segments were 15. 0909,and these were all the polymorphic bands,Polymorphism Information Content（ PIC） of per locus was 0. 8441-0. 8653; the Shannon＇s Information Index（ I） and the Nei＇s Gene Diversities Index（ H） were 0. 1240-0. 3455 and0. 0841-0. 2285,respectively; Coefficient of Gene Differentiation（ Gst） was 28. 78%,the index of Gene Flow（ Nm） an average of 1. 5702,that is,genetic variability mainly exist in individual within population,and moderate gene flow among the different popilations; E. civilis individuals from eight different habitats natural populations were divided into four groups and between the genetic differentiation of interspecific and geographical distance shows a positive correlation.

关 键 词：伞裙追寄蝇 地理种群 ISSR 遗传多样性 遗传分化 

分 类 号：Q968.1[生物学—昆虫学] S433.5[农业科学—农业昆虫与害虫防治]