腧穴针刺对急性期偏头痛大鼠中脑组织SP及其受体NK-1的影响  

Effects of Acupoint Electroacupuncture on Mesencephalon Substance P and Its Receptor Neurokinin-1 in Rats with Acute Migraine

作　　者：唐美霞[1] 王茜[1] 耿俊隆 杜若桑[1] 郑淑美[1] 崔海[1] 

机构地区：[1]首都医科大学,北京100069

出　　处：《中国中医急症》2016年第8期1474-1476,1479,共4页Journal of Emergency in Traditional Chinese Medicine

基　　金：首都中医药研究专项(15ZY21)

摘　　要：目的观察腧穴针刺对偏头痛大鼠中脑P物质(SP)及其受体NK-1 m RNA表达的影响,为探讨针刺治疗急性偏头痛机制提供依据。方法 40只健康Wistar雄性大鼠按随机数字表法分为空白对照组(A组)、模型对照组(B组)、腧穴电针组(C组)、伪电针组(D组)4组,每组10只。各组按设计方案进行造模,和针刺预防治疗后,断头取中脑,应用实时定量PCR技术,测定各组大鼠中脑P物质及其受体NK-1 m RNA的表达量。结果与A组比较,B、C、D组SP m RNA、NK-1 m RNA表达量明显增高(P<0.05)。与B组比较,C、D组SP m RNA、NK-1 m RNA表达量明显降低(P<0.05)。与D组比较,C组SP m RNA、NK-1 m RNA表达量明显降低(P<0.05)。结论针刺能通过下调急性期偏头痛大鼠中脑SP及NK-1基因表达水平,发挥预防治疗偏头痛的作用,且腧穴针刺疗效更佳。Objective：To observe the effect of electroacupuncture on the expression of substance P（SP） and its receptor Neurokinin-1 m RNA in the Mesencephalon in rats with migraine.Methods：40 adult male Wistar rats were equally randomized into 4 groups：the blank group（group A）,the model（group B）,acupoints electroacupuncture（group C）,and non-acupoints electroacupuncture（group D）,10 in each group.After modeling and electroacupunture treatment,all rats were decapitated,and Mesencephalon was removed and SP and receptor Neurokinin-1（NK-1） m RNA expression were detected by real-time quantitative PCR.Results：Compared with A group,the expression of SP m RNA and NK-1 m RNA rose obviously in the other three groups（P 0.05）.Compared with B group,the expression of SP m RNA and NK-1 m RNA dropped obviously in group C and D（P 0.05）.Compared with D group,the expression of SP m RNA and NK-1 m RNA of group C dropped obviously（P 0.05）.Conclusion：Acupoints electroacupuncture can efficiently prevent and treat migraine headache by downregulating gene expression level of SP and NK-1 m RNA,and acupoints electroacupuncture has a better effect.

关 键 词：偏头痛 针刺 P物质 NK-1 

分 类 号：R246[医药卫生—针灸推拿学]