抗菌抗病毒杂合肽C-L的抑菌谱及在模拟胃肠液中稳定性的研究  

Study on antimicrobial spectrum and digestive stability in simulative digestive tract fluid of antibacterial and antiviral hybridpeptide C-L

作　　者：张璐璐[1] 卫旭彪[1] 商婷婷[1] 张日俊[1] 

机构地区：[1]中国农业大学动物科技学院,北京100193

出　　处：《中国科技论文》2016年第12期1340-1343,共4页China Sciencepaper

基　　金：高等学校博士学科点专项科研基金资助项目(20110008110002);"十二五"国家科技支撑计划重点项目(2011BAD26B0403);国家自然科学基金资助项目(31272476)

摘　　要：为分析实验室前期筛选获得的高效抗菌抗病毒杂合肽CecropinA(1-8)-LL37(17-30)(简称C-L)的抑菌谱及在模拟胃肠液中的稳定性,首先利用牛津杯琼脂扩散法,检测其对9种指示菌的抑制效果;然后通过模拟胃肠液处理,观察其抑菌活性变化。结果表明:抗菌抗病毒杂合肽C-L对多种致病菌(金黄色葡萄球菌ATCC43300、金黄色葡萄球菌CVCC1882、金黄色葡萄球菌耐药菌100621、李斯特菌CVCC1599、沙门氏菌CVCC2212、大肠杆菌245)均具有良好的抑制效果,并对益生地衣芽孢杆菌CGMCC1.807无明显的抑菌作用,且抗菌肽C-L经过模拟胃肠液处理后依然保持约90%的抑菌活性。由此表明,该抗菌肽具有良好的抑菌特性及抵抗模拟胃肠液消化的稳定性,对设计优良特性的抗菌肽具有重要的启示,并在健康养殖中具有广阔的应用潜力和价值。In order to analyze antimicrobial spectrum and digestive stability in simulative digestive tract fluid of antibacterial and antiviral hybridpeptide C-L obtained from the previous research in our lab,the inhibitory effect on nine kinds of indicator bacteria was detected by the Oxford cup method and the simulative digestive experiment in vitro was carried out.The experimental results indicate that C-L has good antibacterial effect on several bacteria（Staphylococcus aureus ATCC43300,S.aureus CVCC1882,Multiple-resistant S.aureus 100621,Lester CVCC1599,Salmonella CVCC2212,Escherichia coli 245）,but does not show obvious antibacterial activity on beneficial bacteria Baclicus lincheniformis CGMCC 1.807,and remains about 90% antibacterial activity after the treatment of digestion.In conclusion,antibacterial and antiviral hybridpeptide C-L has good antimicrobial characteristics and stability in the gastrointestinal fliud treatment,which shows great application potential in livestock husbandary production.

关 键 词：饲料微生物学 抗菌抗病毒杂合肽C-L 抑菌谱 抗菌性能 模拟胃肠液 稳定性 

分 类 号：S816.7[农业科学—饲料科学]