青春早期SD雌性大鼠在阿霉素肾病模型过程中生长发育的变化  

Development and growth of female rats in early puberty with adriamycin-induced nephropathy

作　　者：刘俊朝[1] 和永瑞 薛媛媛[1] 孙雯[1] 俞建[1] 汪永红[1] 时毓民[1] 

机构地区：[1]复旦大学附属儿科医院中医科,上海201102

出　　处：《中国中西医结合儿科学》2016年第4期383-386,共4页Chinese Pediatrics of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金(81574022)

摘　　要：目的观察青春早期SD雌性大鼠在阿霉素肾病模型过程中生长发育的变化。方法选取4周龄SPF级雌性SD大鼠10只,随机分为观察组和对照组各5只。观察组大鼠经尾静脉注射阿霉素6.5mg/kg制备肾病模型,对照组大鼠经尾静脉注射等量生理盐水。观察阿霉素注射后1、2、3、4周SD大鼠体质量、子宫湿重、头尾长、胫骨长度变化及测定24h尿蛋白;透射电镜下观察足细胞超微结构变化;放射免疫法分析血清雌二醇(E_2)、促黄体生成素(LH)、促卵泡生成素(FSH)、胰岛素样生长因子1(IGF-1)、胰岛素样生长因子结合蛋白3(IGFBP-3)的变化。结果阿霉素注射后4周青春早期SD雌性大鼠尿蛋白显著高于对照组,差异有统计学意义(P<0.05)。青春期雌性SD大鼠在阿霉素注射后足细胞足突融合逐渐加剧,在阿霉素注射后4周雌性SD大鼠肾小球足细胞足突出现广泛融合。观察组体质量在阿霉素注射后2、3、4周减轻逐渐加剧,与对照组比较差异有统计学意义(P<0.05)。观察组头尾长在阿霉素注射后4周较对照组明显减少,差异有统计学意义(P<0.05)。观察组胫骨长度和子宫湿重在阿霉素注射后2、3、4周后与对照组比较无明显减少,差异无统计学意义(P>0.05)。观察组血清LH、FSH、E_2、IGF-1和IGFBP-3在阿霉素注射后2、3、4周与对照组比较无明显改变,差异无统计学意义(P>0.05)。结论青春早期雌性SD大鼠在阿霉素肾病模型过程中未出现明显的生长发育迟滞,血清生长相关激素和性激素分泌未出现明显降低。Objective To observe the development and growth of female SD rats in early puberty with adriamycin-induced nephropathy.Methods Ten SPF female SD rats,4-week old,were randomly divided into experimental（5rats）and control group（5rats）.The rats in experimental group were injected with adriamycin via the tail vein at the dosage of 6.5mg/kg;the rats in the control group were injected with normal saline via the tail vein.The body weight,uterine wet weight,head-tail length,and tibia length were measured and 24 hurinary protein was determined once a week for 4weeks after injection.Ultrastructural change of podocytes was observed by transmission electron microscope.Serum level of estradiol（E2）,luteinizing hormone（LH）,follicle stimulating hormone（FSH）,insulin-like growth factor 1（IGF-1）,and insulin-like growth factor binding protein 3（IGFBP-3）were measured by radioimmunoassay.Results Urinary protein of the experimental group was significantly higher than that of the control group at the 4th week after adriamycin injection（P〈0.05）.Podocyte foot process effacement was extensive at the 4th week after adriamycin injection.Body weight of the experimental group was more significantly decreased than that of the control group at 2nd,3r d and 4th week after adriamycin injection than that of the control group（P〈0.05）.Head-tail length of the experimental group was significantly reduced compared with the control group（P〈0.05）at the4 th week after adriamycin injection,while tibia length and uterine wet weight showed no significant reduction after adriamycin injection compared with the control group（P〉0.05）.The serum level of LH,FSH,E2,IGF-1and IGFBP-3in the experiment group at the 2nd,3rd and 4th week after adriamycin injection was not significantly changed compared with the control group（P〉0.05）.Conclusion Female SD rats in early puberty with adriamycin-induced nephropathy do not have obvious growth and development retardation;serum growth hormone and sex hormones are not signif

关 键 词：肾病综合征 青春早期 阿霉素肾病 生长发育 大鼠 

分 类 号：R726.9[医药卫生—儿科]