基于ERGO/AuNPs的电化学DNA传感器的制备及应用  

Preparation and applications of electrochemical DNA biosensor based on ERGO/AuNPs

作　　者：许世超[1,2,3] 张雪平[1] 李润兰[1] 张忆一 周晶晶[1] 

机构地区：[1]天津工业大学环境与化学工程学院,天津300387 [2]天津工业大学省部共建分离膜与膜过程国家重点实验室,天津300387 [3]天津工业大学水质安全评价与保障技术工程中心,天津300387

出　　处：《天津工业大学学报》2016年第4期59-63,共5页Journal of Tiangong University

基　　金：天津市自然科学基金资助项目(12JCQNJC086000);天津市自然科学青年基金资助项目(13JCQNJC08200)

摘　　要：制备了一种基于电还原石墨烯(ERGO)、金纳米粒子(Au NPs)修饰玻碳电极的电化学DNA传感器,应用于大肠杆菌O157:H7的快速、灵敏检测.首先将滴加在玻碳电极表面的氧化石墨烯进行电还原,然后通过电沉积方法将金纳米粒子均匀平铺在电极表面.利用金纳米粒子和氨基之间的共价键作用将端氨基修饰的探针DNA固定在电极表面,完成电化学DNA传感器的制备,并对目标DNA进行了定性与定量检测.实验结果表明:所制备的传感器具有良好的选择性、准确性,并且操作简单易行,对目标DNA的检测限为7.735×10^(-13)mol/L,检测范围为1×10^(-12)~1×10^(-8) mol/L.A new electrochemical DNA biosensor was prepared based on glassy carbon electrode（GCE） modified by electri-cally reduced graphene（ERGO） and gold nanoparticles （AuNPs）, which was applied to detect the target DNA of Escherichia Coli O157：H7 rapidly and sensitively. After the electrochemical reduction of the graphene oxide on the surface of GCE, a stable Au-nanoparticles film will be formed on the surface of electrode through elec-trodeposition. Moreover, probe DNA was attached on electrode surface through the covalent band between AuNPs and amino-groups of DNA end. At this point, the electrochemical DNA biosensor was obtained. At last,the qual-itative and quantitative analyses of target DNA were represented. Results showed this fabricated electrochemical DNA biosensor was of good selectivity,sensitivity and easy operation, the limit and range of detection for target DNA were 7.735×10^-13 mol/L and 1 ×10^-12—1 ×10^-8 mol/L, respectively.

关 键 词：电化学DNA传感器 电还原石墨烯 金纳米粒子 大肠杆菌检测 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]