随机引物在黄热病毒核酸检测中的应用  

Detection of yellow fever virus by using random primer

作　　者：田茵[1] 刘翌[1] 王飞[1] 孙福军[1] 张绍福[1] 刘建礼[1] 王正[2] 乌日嘎[2] 祁晓丽[1] 李岩[1] 

机构地区：[1]北京国际旅行卫生保健中心,北京100088 [2]首都机场出入境检验检疫局

出　　处：《中国国境卫生检疫杂志》2016年第3期172-174,共3页Chinese Journal of Frontier Health and Quarantine

摘　　要：目的将随机引物应用于黄热病毒核酸检测,为口岸快速准确地检测黄热病毒提供技术支持。方法采用随机引物和特异性引物进行逆转录,分别用自行设计的引物、探针和参考文献的引物、探针,用实时荧光PCR方法对6份黄热病毒阳性尿液样本进行黄热病毒核酸检测,并对检测结果进行比较。结果与特异性引物逆转录相比,随机引物逆转录的实时荧光PCR Ct值差别不显著(F值=0.11,P>0.05)。结论随机引物可直接应用于黄热病毒检测,也可在口岸传染病监测中发挥其他作用。Objective To detect yellow fever virus nucleic acid by using random primer,and to provide technical support to rapid detection of yellow fever virus. Methods A total of 6 urine samples of 3 imported cases of yellow fever infection were detected by Real-time PCR assay. The random primer and specific primer were used to reverse transcript,Real-time PCR assay was conducted by using primers and probe designed and referenced,respectively. The Ct values had been analyzed. Results The amplification efficiency of Real-time PCR reactions by using random primer in reverse transcription had no significant difference to that by using specific primer （F value=0.11,P〉 0.05）. Conclusion Random primer could be used to detect yellow fever virus directly,and had some other important roles in infectious diseases surveillance at ports.

关 键 词：黄热病毒 随机引物 实时荧光RT-PCR 

分 类 号：R373.3[医药卫生—病原生物学] R466.12[医药卫生—基础医学]