大鼠应激性心肌病模型及其复发模型的制备  

Preparation for rat model of stress-induced cardiomyopathy and its recurrence model

作　　者：蔡伟文[1,2] 杜志君[1] 查道刚[1] 

机构地区：[1]南方医科大学南方医院心内科,广州510515 [2]武警广东省边防总队机关门诊部,广州510660

出　　处：《岭南心血管病杂志》2016年第3期315-319,共5页South China Journal of Cardiovascular Diseases

摘　　要：目的探索应用去甲肾上腺素（norepinephrine,NA）制备大鼠应激性心肌病（stress--induced cardiomyopathy,SIC）模型的方法,并探讨应用其制备SIC复发模型的可能性。方法 65只雄性SD大鼠（300～350 g）用于本研究。首先取30只按随机数字表法随机分成5个组,分别接受NA 1、2、3、4、5 mg/kg腹腔注射用于探索最适剂量。给药90 min后采用超声心动图观察室壁运动,以出现左心室功能障碍,心尖节段（典型）或非心尖节段（非典型）室壁运动失活判定成功造模。确定NA造模最适剂量后,另取30只接受单一该剂量NA腹腔注射进行造模及1周后复发造模,5只用于正常对照。结果 NA剂量≥2 mg/kg可诱发出节段性室壁运动异常的SIC模型,且1周内可自行恢复正常;其中4 mg/kg组成功率高达66.67%,为最适剂量。30只最适剂量NA造模大鼠中,造模成功率为53.3%（典型SIC 3只,非典型SIC13只）,1周后其中10只非典型SIC大鼠复发模型诱导成功率为30.0%,且转变为典型SICo结论应用NA腹腔注射可成功制备初发、复发SIC模型,制备方法简单精确,重复性好,模型符合SIC患者的临床特征,支持儿茶酚胺毒性效应为SIC的发病机制。Objectives To explore how to prepare the rat model of stress-induced cardiomyopathy （SIC） with norepinephrine （NA）, and to explore the possibility of preparation for its recurrence model. Methods We used 65 male SD rats （300-350 g） in this study. Of these rats, 30 animals were randomly divided into 5 groups receiving different doses of NA （1,2,3,4,5 mg/kg, n=6 respectively） intraperitoneally to study the optimum dose. Echocardiography was used to measure wall motion 90 min post administration. Criteria for successful modeling was occurrence of left ventricular dysfunction accompanied by apical （＂typical＂） or non-apical （＂atypical＂） akinesis. After determining the optimum dose of NA, another 30 rats were injected with single dose intraperitoneally to induce SIC, and 1 week later to induce recurrence model. Another 5 rats were selected as normal control. Results NA at doses ≥ 2 mg/kg could induce SIC- like regional akinesia in rats and it could be completely resolved within 1 week. Success rate of 4 mg/kg group achieved 66.67% and 4 mg/kg was the optimum dose. Success rate of the 30 rats with the optimum dose of NA was 53.3% （3 typical SIC rats, 13 atypical SIC rats）. After 1 week, success rate of recurrent SIC model in 10 atypical SIC rats was 30%, and they all transformed into typical SIC. Conclusions Models of primary and recurrent SIC can be successfully prepared by intraperitoneal injection of NA. The establishing method is simple and reproducible. The models arc consistent with the patient＇s clinical characteristics of SIC, supporting the patbogenesis of catecholamine- mediated cardiotoxicity.

关 键 词：应激性心肌病 TAKO-TSUBO心肌病 非典型应激性心肌病 应激性心肌病复发 去甲肾上腺素 

分 类 号：R542[医药卫生—心血管疾病]