利用超声彩色多普勒技术评价大鼠腹主动脉狭窄模型  

Evaluation of a rat model of abdominal aortic constriction by the technology of color Doppler echocardiography

作　　者：符永恒[1] 张梦珍[1] 谭卫萍[2] 朱杰宁[1] 刘晓颖[1] 林秋雄[1] 杨敏[1] 谭虹虹[1] 单志新[1] 

机构地区：[1]广东省心血管病研究所广东省人民医院(广东省医学科学院)医学研究中心,广州510080 [2]广东省人民医院(广东省医学科学院)图书馆,广州510080

出　　处：《岭南心血管病杂志》2016年第4期465-469,486,共6页South China Journal of Cardiovascular Diseases

基　　金：国家自然科学基金项目(项目编号:81070102;81270222;81273516);广东省自然科学基金重点项目(项目编号:S2011020005911)

摘　　要：目的利用超声彩色多普勒技术对构建的大鼠腹主动脉缩窄(abdominal aortic constriction,AAC)模型进行检测,评价动物模型的心脏结构、功能变化。方法以0.3 mm银夹缩窄双侧肾动脉上段(约0.5 cm处)的腹主动脉制备压力超负荷大鼠模型36只,按时间分为AAC-2周组、AAC-4周组和AAC-8周组及假手术(Sham)组(每组12只)。建模后2周、4周和8周时,用Vevo 2100型动物微小超声心动图仪进行M-mode超声检测心功能指标,如射血分数(ejection fraction,EF)、左心室短轴缩短率(fractional shortening,FS)、左心室舒张期前壁厚度(left ventricular end-diastolic anterior wall thickness,LVAWd)、左心室收缩期前壁厚度(left ventricular endsystolic anterior wall thickness,LVAWs)、左心室舒张期后壁厚度(left ventricular end-diastolic posterior wall thickness,LVPWd)、左心室收缩期后壁厚度(left ventricular end-systolic posterior wall thickness,LVPWs)、左心室舒张末期内径(left ventricular end-diastolic internal dimension,LVEDd)、左心室收缩末期内径(left ventricular endsystolic internal dimension,LVEDs);和超声彩色多普勒测量腹主动脉缩窄前、后的血流量,二尖瓣E/A值;测量左心室质量指数(left ventricular mass index,LVMI)等。结果(1)同Sbam组比较,AAC-2周组的EF、FS、LVEDd、LVEDs明显降低(P<0.01),而LVAWd、LVAWs、LVPWd、LVPWs均明显增加(P<0.05);腹主动脉缩窄前段血流量与管腔增大,缩窄后段血流量和管腔显著变小;且出现E/A值和LV MI改变明显(P<0.01),AAC-2周组左心室肥厚明显。(2)同AAC-2周组比较,AAC-4周和AAC-8周组EF、FS、LVAWd、LVAWs、LVPWd、LVPWs明显降低,而LVEDd、LVEDs明显增加,且缩窄后段血流速度、血流量明显变小,而E/A值及LVMI增加明显(P<0.05)。AAC-4周组与AAC-8周组心脏体积、左心室腔明显增大呈心力衰竭病变。结论利用动物微小彩色多普勒技术能直观、准确地检测和评价以银夹缩窄腹主动脉的大鼠模型;AAC模型Objectives To determine the changes of cardiac structure and function in a rat model of abdominal aortic constriction （AAC） by color Doppler echocardiography assay. Methods A rat model of AAC was established by fixing a 0.3 mm silver clip at the bilateral renal artery （located at about 0.5 cm from the top）. Thirty-six survived rats were randomized into 2-week AAC （AAC-2W） group, AAC-4W group, AAC-SW group and sham operation （Sham） group （12 in each group）. At 2 weeks, 4 weeks and 8 weeks after AAC surgery, the rats underwent two-dimensional （2D）-guided M-mode transthoracic echocardiography by tiny echocardiography instrument Vevo 2100. The cardiac function variables, including ejection fraction （EF）, fractional shortening （FS）, left ventricular end-diastolic anterior wall thickness （LVAWd）, left ventricular end-systolic anterior wall thickness （LVAWs）, left ventricular end-diastolic posterior wall thickness （LVPWd）, left ventricular end-systolic posterior wall thickness （LYPWs）, left ventricular end- diastolic internal dimension （LVEDd） and left ventricular end-systolic internal dimension （LVEDs） were determined. Blood flows before and after AAC surgery and mitral E/A ratios were measured by color Doppler assay. Left ventricular mass index （LVMI） was also measured. Results （1） Compared with sham group, variables of EF, FS, LVEDd and LVEDs were significantly lower （P〈0.01）, while LVAWd, LVAWs, LVPWd and LVPWs significantly increased （P〈 0.05） in AAC-2W group. Anterior blood flow before AAC surgery and vascular lumen increased, while posterior blood flow after AAC and vascular lumen significantly decreased, with significant changes of E/A ratios and LVMI （P〈0.01）. Left ventricular hypertrophy appeared in 2 weeks after AAC surgery. （2） Compared with AAC-2W group, EF, FS, LVAWd, LVAWs, LVPWd and LVPWs significantly reduced, while LVEDd and LVEDs significantly increased in AAC- 4W group and AAC-SW group. Posterior blood flow velocity

关 键 词：超声仪彩色多普勒 腹主动脉缩窄 心肌肥厚 心力衰竭 

分 类 号：R543.1[医药卫生—心血管疾病]