虫害诱导果实挥发物对蝇蛹金小蜂趋性行为的影响及其成分初步鉴定  

Attraction of Pachycrepoideus vindemmiae by herbivore-induced fruit volatiles to and extraction and GC-MS identification of the active components

作　　者：赵海燕[1] 梁广文[2] 陆永跃[2] 

机构地区：[1]海南省农业科学院植物保护研究所/海南省植物病虫害防控重点实验室,海口571100 [2]华南农业大学昆虫学系,广州510642

出　　处：《植物保护》2016年第4期83-88,共6页Plant Protection

基　　金：海南省自然科学基金(20153065);海南省农业科学院农业科技创新专项经费(CXZX201419)

摘　　要：为了解果实挥发物在蝇蛹金小蜂寻找寄主过程中的作用,本文采用"Y"形嗅觉仪测试了健康果实及虫害诱导果实挥发物对蝇蛹金小蜂的引诱趋性。测定结果表明,虫伤1d的番石榴和阳桃果实挥发物对蝇蛹金小蜂雌蜂具有显著的引诱作用。进而选取了虫伤1d的番石榴和阳桃果实,利用固相微萃取法对其挥发物进行了收集,并用GC-MS对收集到的挥发物进行了初步鉴定。鉴定结果表明,虫伤1d的番石榴果实挥发物中共检测到8种化合物,多是萜烯类化合物,其中反式-石竹烯的相对含量最高,其次是二十二烷醇;虫伤1d的阳桃果实挥发物成分中共鉴定出16种物质,主要为烷烃类、萜烯类、酯类及酮类,其中α-紫罗兰酮含量最高,其次为11-硬酯炔酸甲酯。In order to understand the roles of fruit volatiles and herbivore-induced fruit volatiles in host searching of Pachycrepoideus vindemmiae （Rondani）, the orientation responses of P. vindemmiae to the volatiles under different treatments were studied with a ＂Y＂-tube olfactometer. The female adults of P. vindernmiae were significantly attracted by the volatiles from guava fruits damaged by larvae for one day （DG-ld） and carambola fruits damaged by larvae for one day （DC-1d）. Then, the chemical constituents from the DG-ld and DC-ld were analyzed by gas chromatography-mass spectrometry （GC-MS） with headspace solid-phase microextraction （SPME）. The results showed that eight compounds were obtained and the main compounds were terpenes and sesquiterpenes from the volatiles emitted from LDGF-1D. The relative content of trans-caryophyllene was the highest, followed by 1-docosanol from DG-ld volatiles. In addition, sixteen compounds were identified from the volatiles emitted from DC-1d, including alkanes, terpenes, esters and ketone. The relative content of α-lonone was the highest, followed by 11-octadecynoic acid methyl ester.

关 键 词：虫害诱导 果实挥发物 蝇蛹金小蜂 

分 类 号：S476.3[农业科学—农业昆虫与害虫防治]