比较小肠黏膜下层基质放置在腹壁不同层次的转归  

Compare the outcomes of small intestinal submucosa after being implanted in different layers of rat's abdominal wall

作　　者：梁析[1] 汤睿[2] 冯学艺[3] 王新[3] 刘正尼[2] 朱晓强[2] 王兵[3] 

机构地区：[1]上海交通大学附属第九人民医院胸外科,200011 [2]同济大学附属东方医院普外科,200085 [3]上海交通大学附属第九人民医院普外科、疝与腹壁外科,200011

出　　处：《中华疝和腹壁外科杂志（电子版）》2016年第4期255-260,共6页Chinese Journal of Hernia and Abdominal Wall Surgery(Electronic Edition)

摘　　要：目的观察猪小肠黏膜下层基质(PSIS)放置在SD大鼠腹壁不同层次的转归。方法大小1 cm×2 cm PSIS膜片被放置在SD大鼠腹壁的不同层次(皮下、腹膜前、腹膜后),分别于术后1、2、4、9周取材,观察动物大体情况和组织学变化。结果术后动物均存活,均无伤口感染及血肿形成,3例浆液肿均出现在腹膜前组。三组均未见明显免疫排斥反应,但在1、2及4周时间点上,腹膜后组巨噬细胞计数强于其他两组(P<0.05),9周时间点上无明显差异(P>0.05)。1周时腹膜前组的血管化优于其他两组(P<0.05),余时间点均无明显差异(P>0.05)。组织重塑情况,4周时腹膜后组新生胶原排列相对其他两组较紊乱、疏松。9周时腹膜前组新生胶原量相对较多,皮下组胶原排列连续性、致密性相对较好,余时间点三组无明显差异(P>0.05)。结论 PSIS是一种可降解、具有良好生物相容性和组织再生能力的生物材料,植入腹壁各层次后各有优缺点。Obejective This animal study was underwent to investigate the outcomes of porcine small intestinal submucosa （PSIS） after being implanted in different layers of rat＇s abdominal wall, Methods The 1 cm x 2 cm PSIS meshes were implanted in different layer of abdominal wall in rats （ Onlay, Sublay and Underlay）. The rats were harvested at week 1, 2, 4, and 9 after implantation. General state and histological responses of the animals were observed. Results All animals survived without infection and hematoma formation. Three cases of seroma were found only in Sublay group. No obvious reject reaction was observed in all three groups, while the macrophages invasion in Underlay group was stronger than that in the other two groups at 1, 2 and 4 week after operation （P 〈 0.05 ）. The revascularization of abdominal wall was better in Sublay group compared to the other two groups at 1 week after operation （P 〈 0.05）, there was no significant statistical difference at 2, 4, and 9 week after operation （P 〉 0.05 ）. Collagenous tissue at the Underlay group was shown slightly disorganized when comparing with the other two groups at 4 week. Better organized collagen was observed at onlay group compared to the other two groups and the collagen amount was abundant at sublay group compared to the other two at 9 week. Conclusions As a biological material, PSIS is biodegradable, biocompatible and is good for tissue regeneration. The outcomes of PSIS after being implanted in different layer were diverse due to the variety of microenvironments. Further study will be performed to investigate the details to improve the clinical outcomes.

关 键 词：腹壁 肠黏膜 基质 

分 类 号：R318.08[医药卫生—生物医学工程] R656.3[医药卫生—基础医学]