当归/蒺藜不同配比对过氧化氢诱导实验性白癜风豚鼠的治疗作用  

Effect of Different Ratio of Angelica and Tribulus on Experimental Vitiligo Guinea Pig Model

作　　者：张丽宏[1] 安丽凤[1] 黄敬文[1] 鲁光宝[1] 刘海洲[1] 王佳娜[1] 王兴焱[1] 王英莲[1] 

机构地区：[1]黑龙江中医药大学佳木斯学院,黑龙江佳木斯154007

出　　处：《辽宁中医药大学学报》2016年第8期36-38,共3页Journal of Liaoning University of Traditional Chinese Medicine

基　　金：黑龙江省教育厅科学技术研究项目(12531626)

摘　　要：目的:考察当归蒺藜不同配比对实验性白癜风豚鼠的治疗作用。方法:采用过氧化氢脱色法制备白癜风豚鼠模型,当归蒺藜液治疗50 d后,对受试区皮肤的恢复情况进行疗效评估,应用ELISA法检测血清中MAO、Ch E和TYR活性,L-DOPA氧化法测定皮肤TYR活性,免疫组化法观察皮肤黑色素的分布情况。结果:应用当归蒺藜液处理后,受试区皮肤逐渐开始着色,血清MAO活性下降,Ch E和TYR活性均有所提高,皮肤TYR活性增加,黑色素的分布明显增多,均有统计学意义,以当归蒺藜(2:1)组最佳(P<0.01)。结论:当归蒺藜不同配比对实验性白癜风豚鼠具有一定的治疗作用,其作用机制可能通过调节MAO、Ch E和TYR活性,增加皮肤黑色素的分布,从而促进皮肤着色实现的。Objective：Investigate the effect of different ratio of Angelica and Tribulus on experimental vitiligo guinea pig model. Methods：Vitiligo guinea-pig model was prepared by hydrogen peroxide bleaching,after treatment of different ratio of Angelica and Tribulus Liquid for 50 days,test region skin recovery and evaluate therapeutic effects,detect plasma Ch E and tyrosinase by ELISA method,detect tyrosinase of skin by L-DOPA oxidation,observe the distribution of melanin in the skin of each layer by immunohistochemical method. Results：After the treatment of Angelica and Tribulus Liquid,the experimental area skin gradually began to coloring,the plasma MAO activity was decreased,Ch E and tyrosinase activity were increased,tyrosinase of skin were increased,significantly increased the distribution of melanin in the skin,were statistically significant,with Angelica Tribulus terrestris（2：1）group（P〈0.01）.Conclusion：Different compatibility proportion of Angelica and Tribulus have a certain therapeutic effect on experimental vitiligo guinea pig model,which may be through the regulation the plasma MAO、Ch E and tyrosinase activity,increased melanin in the skin of the distribution,promotion of skin color.

关 键 词：当归 蒺藜 白癜风 

分 类 号：R758.41[医药卫生—皮肤病学与性病学]