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机构地区:[1]上海理工大学上海市现代光学重点实验室,上海200093 [2]上海科源电子科技有限公司,上海201101
出 处:《电子科技》2016年第8期7-9,13,共4页Electronic Science and Technology
基 金:上海市教育委员会青年教师基金资助项目(ZZslg15015)
摘 要:生物摇床是一种实验室常见的微生物、细菌与细胞培养仪器。传统摇床仅能为被培养物质提供良好的培养环境,却无法获知其实时生长状况。为此,文中在浊度法的基础上设计了一种在线吸光度检测电路。该电路由LED控制电路、光电转换电路、放大电路与A/D采样电路4个模块组成,引入该电路系统的摇床能在生物培养的同时实现实时生物浓度检测。通过搭建相关实验平台,对该电路检测的准确性及稳定性做了系统验证。实验结果表明,设计的培养监测电路的吸光度检测误差控制在3%以内,能准确地反应出当前被培养物质的吸光度值,即该电路能实现生物摇床的在线监测培养。Biological shaking incubator is a common laboratory instrument often used to isms, bacteria and cells. Traditional incubators fail to provide real-time information on material cultivate microorgan- growth. A circuit of online optical density detection based on turbidity method is designed. The circuit, consisting of the LED controlling circuit, the photoelectric conversion circuit, the amplifier and the A/D sample circuit, realizes the real optical densi- ty detection during cultivation. Relative platform has been set up to verify the accuracy and stability of the optical density detection circuit. The error between the optical density value detected by optical density detection circuit and the average one in different states is shown to be less than 3%, indicating that the circuit reflects the optical density value of the cultured materials in real time accurately.
分 类 号:TN710[电子电信—电路与系统]
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