HPLC-ESI-MS^n法鉴定大鼠口服白芍提取物后血清及脑内的化学成分  

Identification of chemical components of Paeoniae Alba Radix extracts in rat serum and brain after oral administration by HPLC-ESI-MS^n

作　　者：李芳[1] 朱付全 宋春红[3] 王杰琼[3] 张惠云[3] 乔明琦[3] 

机构地区：[1]北京市丰台区妇幼保健院药剂科,北京100069 [2]山东省平邑县中医医院老年病科,山东平邑273300 [3]山东中医药大学中医药经典理论教育部重点实验室,山东济南250355

出　　处：《中草药》2016年第14期2475-2481,共7页Chinese Traditional and Herbal Drugs

基　　金：国家自然科学基金资助项目(81173163;81173151)

摘　　要：目的定性分析白芍提取物在大鼠血清及脑组织中的化学成分。方法大鼠ig给予白芍提取物后,采集血液并分离脑组织部位,采用HPLC-ESI-MSn法检测入血及分布于脑组织中的化学成分。结果通过相应保留时间和质谱数据,以及与文献资料及对照品比对,在大鼠含药血清中共检测到34个化学成分,其中8个为新代谢产物;在全脑组织中共检测到3个化学成分,其中1个为新代谢产物。结论建立的HPLC-ESI-MSn方法有助于阐明白芍提取物抗抑郁作用的药效物质基础。Objective Qualitative analysis of chemical components in Paeoniae Alba Radix extracts（PARE） in rat serum and brain.Methods After ig administration of PARE, rat blood and brain tissues were collected. The chemical components in serum and brain were analyzed by HPLC-ESI-MSn. Results By comparing retention time, MS and MSn spectra with data in the literature and reference standards, a total of 34 compounds including eight new metabolites were identified in serum, and three components including one new metabolite were identified in rat brain. Conclusion This research will contribute to elucidate the pharmacodynamic material basis of antidepressant effect of PARE.

关 键 词：白芍提取物 血清 脑 HPLC-ESI-MSn 代谢产物 

分 类 号：R285.5[医药卫生—中药学]