丹参沼泽红假单胞菌转化液中5个酚酸类成分含量的同时测定  

Simultaneous Determination of Five Phenolic Acids in Salvia Miltiorrhizha Bunge Extraction Bioconverted by Rhodopseudomonas Palustris

作　　者：张源[1] 关春杰[1] 冀蔚佳 耿子颖[1] 白小红[1] 杨官娥[1] 

机构地区：[1]山西医科大学药学院,山西太原030001

出　　处：《中医药导报》2016年第17期43-46,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：太原市科技项目人才专项明星专题(120247-08);国家自然科学基金项目(81173473);山西省自然科学基金项目(2010011048-2);2012年山西省高等学校大学生创新创业训练项目(201231)

摘　　要：目的：建立HPLC法同时测定丹参沼泽红假单胞菌转化液中丹参素、原儿茶醛、迷迭香酸、丹酚酸B和丹酚酸A5种成分的含量，并和丹参对照液进行比较。方法：采用HPLC法，色谱柱为岛津InertSustainC18柱（4．6mm×250mm，5μm）；甲醇-0．1％磷酸水梯度洗脱；检测波长为287nm，流速为0．8mL·min^-1，柱温为26℃，进样量为25μL。结果：丹参素、原儿茶醛、迷迭香酸、丹酚酸B和丹酚酸A分别在20-1000μg·mL^-1，5-200μg·m^-1，5~200μg·mL^-1，5～1000μg·mL^-1，10～200μg·mL^-1范围内呈良好的线性关系（r〉0．9997）。加样回收率分别为101．31％，101．13％，98．81％，103．47％，99．06％。结论：该方法准确可靠，具有良好的精密度、重复性、稳定性和回收率，可以用于丹参沼泽红假单胞菌转化液中5个酚酸类成分含量的同时测定，为丹参沼泽红假单胞菌转化液的进一步研究奠定基础。Objective： To establish a HPLC method for determination tanshinol, protocatechualdehyde, ros- marinic acid, salvianolic acid B and salvianolic acid A in Salvia miltiorrhizha Bunge extraction bioconverted by Rhodopseudomonas palustris, and to compare with salvia miltiorrhiza control liquid. Methods： HPLC was applied on a SHIMADZU InertSustain C18 column （4.6mm×250 mm, 5 μm） with methanol-0.1% phosphoric acid water as mobile phase by gradient elution. The detection wavelength was 297 nm, and the flow rate was 0.8 mL·min ^-1; the column temperature was 26 ℃ and the injection volum was 25μL. Results： Tanshinol, protocatechualdehyde, rosmarinic acid, salvianolic acid B and salvianolic acid A presented a good linearity （r〉0.9997） in the concen- tration ranges of 20~1000 μg·mL^-1, 5-200 μg·mL^-1, 5-200 μg·mL^-1, 5~1000 μg·mL^-1 and 10-200 μg·mL^-1 re- spectively. The recovery was 101.31%, 101.13%, 98.81%, 103.47% and 99.06% respectively. Conclusions： The methoed is accurate and reliable, with good precision, repeatability, stability and recovery. It can be used to de- termine the five phenolic acids in Salvia miltiorrhizha Bunge extraction bioconverted by Rhodopseudomonas palustris simultaneously. The study provides scientific basis for further research of S. miltiolvhizha Bunge bio- transformed by Rhodopseudomonas palustris.

关 键 词：丹参 光合细菌 沼泽红假单胞菌 酚酸类成分 含量测定 

分 类 号：R284.1[医药卫生—中药学]