正交试验法优化消肿止痛中药复方醇提工艺  

To Optimize Alcohol Extraction Process of Swelling-Dispersing and Pain-Relieving Chinese Medicine Compound by Orthogonal Test

作　　者：吴涵[1] 张建环[1] 王凌[1] 吴雪茹[1] 

机构地区：[1]广州中医药大学附属骨伤科医院,广东广州510240

出　　处：《中医药导报》2016年第17期47-48,52,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：2014年广东省中医药局科研课题(20141112);2015年中医药强省专项基金医院中药制剂建设项目

摘　　要：目的:通过正交试验法优化消肿止痛中药复方的醇提工艺。方法:建立HPLC法,以乙醇浓度、乙醇用量、回流时间、回流次数为考查因素,提取液中大黄素含量为评价指标,进行正交试验,采用SPSS统计软件分析实验结果。结果:得到最佳提取工艺为10倍量90%乙醇提取120 min,提取3次。结论:通过正交试验法优化消肿止痛中药复方的醇提工艺后可得到更佳的提取效果,进而提高复方的临床疗效。Objective： To optimize alcohol extraction process of Swelling-Dispersing and Pain-Relieving Chinese medicine compound by orthogonal test. Methods： We established a quantitative method for emodin by HPLC. With ethanol concentration, ethanol volume, extraction time, reflux times as factors, emodin content in the extract as the evaluation index, the orthogonal test was carried out, results of which were analyzed by SPSS. Results： The optimum extraction process is obtained as 10-fold times of 90% alcohol refluxing 120 min, and 3 times. Conclusion： Better extraction effects were got of Swelling-Dispersing and Pain-Relieving Chinese medicine compound by orthogonal test, which means better clinical effects.

关 键 词：大黄素 消肿止痛 醇提工艺 正交试验 HPLC法 

分 类 号：R284.2[医药卫生—中药学]