分光光度法间接测定利血生片剂含量  

Determination of leucogen by indirect ultraviolet spectrophotometry

作　　者：焦更生[1] 郎惠云[1] 张维萍[1] 

机构地区：[1]西北大学化学系,陕西西安710069

出　　处：《西北大学学报（自然科学版）》2002年第4期374-376,共3页Journal of Northwest University（Natural Science Edition）

摘　　要：利血生在碱性介质中的分解产物在 2 2 0± 1 nm处有强的吸收 ,采用分光光度法间接测定利血生的含量。结果表明 :在线性范围 1 0 .0～ 1 0 0 .0 μg/m L内 ,表观摩尔吸光系数 ε=1 .1 3× 1 0 4L/mol· cm,回收率为 1 0 3.0 %～ 1 0 5 .5 % ;与滴定法相比 ,相对偏差小于 1 .0 % ;此法具有灵敏度高、线性范围宽、操作简便。The amount of leucogen in tablets has been measured by ultraviolet spectrophotometry. It is based on the strong absorption in 220±1nm when leucogen is resolved in alkaline solution. The results show that the relative deviation between this method and titrimetry is less than 1.0%, the linear range is 10.0～100.0 μg/mL, ε is 1.13×10 4 L/mol·cm and the recovery is 103.0%～105.5%.This method is simpler and more rapidly than titrimetry used in pharmaceutical factories at present.

关 键 词：分光光度法 间接测定 利血生片剂 吸光度 含量测定 抗贫血药 

分 类 号：R973[医药卫生—药品] R927.2[医药卫生—药学]